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Merck

P5899

Poly-ʟ-Lysine Hydrobromide

synthetic, mol wt ≥300,000, powder, γ-irradiated, suitable for cell culture, BioXtra

Sinónimos:

PDL HBr

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About This Item

Número CAS:
25988-63-0
UNSPSC Code:
12352202
eCl@ss:
32160406
PubChem Substance ID:
24898718
NACRES:
NA.75
MDL number:
MFCD00237230

Nombre del producto

Poli-L-lisina hydrobromide, mol wt ≥300,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

SMILES string

Cl.NCCCCC(N)C(O)=O

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

InChI key

WBSCNDJQPKSPII-KKUMJFAQSA-N

biological source

synthetic (organic)

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

mol wt

≥300,000

packaging

pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

surface coverage

4 μg/cm2

impurities

<10% water (by Karl Fischer)

solubility

H2O: soluble 50 mg/mL, clear to very slightly hazy, colorless to faintly yellow

shipped in

ambient

storage temp.

−20°C

Quality Level

200

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Disclaimer

Las disoluciones estériles son estables durante un máximo de 2 años cuando se conservan entre 2 y 8°C. Deben guardarse desecadas a -20°C.

Other Notes

La poli-L-lisina es una polímero de aminoácido con carga positiva con aproximadamente un HBr por residuo de lisina. El hidrobromuro permite a la poli-L-lisina estar en una forma cristalina hidrosoluble. Puede encontrarse una pequeña cantidad del producto en la estructura beta porque el HBr interfiere en el enlace de hidrógeno entre el grupo amino y los grupos carboxilo o los grupos que contienen N u O.
For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.

Analysis Note

Molecular weight based on viscosity. Also assayed by MALLS.

Application

Poly-L-lysine hydrobromide has been used to coat:
  • 2-well plates before seeding muscle cells in a research study on rainbow trout myotube formation
  • flat bottom 96-well plates for in vitro neuronal stem cell differentiation
  • T75 flasks for primary astrocytic cultures

Biochem/physiol Actions

Poly-L-lysine serves as a coating substance to ensure better attachment of cells to culture surfaces. Research shows that plates coated with poly-L-lysine (PLL) demonstrated an accelerated cell growth rate. Culturing mesenchymal stem cells (MSCs) on PLL-coated substrates has been found to safeguard the stemness properties of these cells and inhibit senescence. PLL-coated plates have demonstrated enhanced MSC growth and up-regulated the expression of genes associated with cell adhesion, differentiation, proliferation, and signaling. Moreover, porous PLL-coated surfaces have shown improvements in cell proliferation and the osteogenic differentiation of MSCs.

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000315801
SLCL5836
0000315801
SLCL5836
SLCF0030

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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Poly-L-lysine Prevents Senescence and Augments Growth in Culturing Mesenchymal Stem Cells Ex Vivo
June Seok Heo, et al.
BioMed Research International (2016)
Gustavo Castro et al.
ISRN ophthalmology, 2013, 752161-752161 (2014-02-22)
Purpose. The purpose of this work was to evaluate the retinal integration and differentiation of neurospheres formed by stem cells and mouse neural progenitor cells injected intravitreally in mice eyes with retinal injury. Methods. Eight male C57BL mice, 8 weeks
Sonja Stadler et al.
BMC cell biology, 5(1), 44-44 (2004-11-24)
Between cell divisions the chromatin fiber of each chromosome is restricted to a subvolume of the interphase cell nucleus called chromosome territory. The internal organization of these chromosome territories is still largely unknown. We compared the large-scale chromatin structure of
Polylysine for skin regeneration: A review of recent advances and future perspectives
Payam Zarrintaj, et al.
Journal of Separation Science, 5(7) (2021)
Direct addition of poly-lysine or poly-ethylenimine to the medium: A simple alternative to plate pre-coating
Alexander Faussner, et al.
PLoS ONE, 17(7) (2022)
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