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Merck

S5265

Streptolysin O from Streptococcus pyogenes

lyophilized powder, Protein ~3 % by Lowry, 25,000-50,000 U/vial

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Número CAS:
98072-47-0
UNSPSC Code:
12352202
EC Number:
308-500-3
NACRES:
NA.85
MDL number:
MFCD00132389

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Nombre del producto

Streptolysin O from Streptococcus pyogenes, lyophilized powder, Protein ~3 % by Lowry, 25,000-50,000 U/vial

form

lyophilized powder

specific activity

25,000-50,000 U/vial

mol wt

native 69 kDa

composition

Protein, ~3% Lowry

storage condition

(Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place.)

color

white

solubility

H2O: soluble 1 mg/mL

antibiotic activity spectrum

neoplastics

mode of action

cell membrane | interferes

storage temp.

2-8°C

Quality Level

200

Gene Information

Streptococcus pyogenes MGAS8232 ... slo(993428)

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Other Notes

Keep container tightly closed in a dry and well-ventilated place. Keep in a dry place
One unit will cause 50% lysis of 2% red blood cell suspensions in phosphate buffered saline, pH 7.4, after incubation at 37 °C for 30 min.

Packaging

25KU

Application

Permeabilizes membranes to permit cellular uptake of large or charged molecules.
Streptolysin O is a toxin secreted by Streptococcus pyogenes and is a prototype member of poreforming bacterial cytolysins. It is used permeabilize cell membranes to permit cellular uptake of large or charged molecules. It is used to study macromolecule delivery. It is a potential anticancer agent and is used to study suicide cancer gene therapy.
Used to create holes (reversible pores) in cholesterol-enriched plasma membranes of eukaryotic cells allowing selective permeabilization with molecules such as proteins (up to 100 kDa) and siRNAs.

Biochem/physiol Actions

Streptolysin O possesses a single polypeptide chain with a molecular weight of f62 kDa. Streptolysin O binds to membrane cholesterol and oligomerizes to create a ring structure that consists of 45 to 50 units. The ring structure inserts into the membrane to make a large pore (25 to 30 nm), which DNA, RNA, peptides and proteins may pass. It is thiol-activated. It is inhibited by allicin, an active component of garlic.
Thiol-activated toxin that permeabilizes animal cell membranes. The protein binds as a monomer to membrane cholesterol and subsequently polymerizes into large arc- and ring-shaped structures surrounding pores of >12 nm.
Thiol-activated toxin that permeabilizes animal cell membranes..

Physical form

Lyophilized powder containing Tris buffer salts, sodium azide, PMSF, and EDTA.

signalword

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2

Clase de almacenamiento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000518546
0000518546
0000404460
0000404460
0000192753

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Wan Seok Yang et al.
Molecular cancer therapeutics, 5(6), 1610-1619 (2006-07-05)
We cloned the streptolysin O gene from the Streptococcus pyogenes genome and tested the possibility of using it as an anticancer reagent. Transient transfection of the streptolysin O gene efficiently killed 293T cells after 12 hours of transfection as determined
Chen Chen et al.
Nanoscale, 10(11), 5154-5162 (2018-03-02)
Exosomes are small membrane vesicles secreted by a wide variety of cells. Studies have demonstrated that exosomal miRNAs can influence the biological processes of recipient cells. Therefore, direct imaging and tracking of exosomal miRNAs in living recipient cells are essential
Neurotransmitter interactions related to central dopamine neurons.
K G Lloyd
Essays in neurochemistry and neuropharmacology, 3, 129-207 (1978-01-01)
Stefanie Klar et al.
Journal of occupational medicine and toxicology (London, England), 16(1), 32-32 (2021-08-14)
Workers of agriculture and intensive life stock farming are exposed to highly contaminated workplaces. Bioaerosol exposures are suspected to trigger respiratory health effects of the workers. So far, risk evaluation of bioaerosols has been assessed through the infectivity of comprising
John Popovich et al.
Frontiers in bioengineering and biotechnology, 8, 527-527 (2020-06-26)
Pore-forming and hemolytic toxins are bacterial cytotoxic proteins required for virulence in many pathogens, including staphylococci and streptococci, and are notably associated with clinical manifestations of disease. Inspired by adsorption properties of naturally occurring aluminosilicates, we engineered inexpensive, laboratory-synthesized, aluminosilicate
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Data Sheet - S5265

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