T2885

3,3′,5,5′-Tetrametilbencidina

Name: 3,3′,5,5′-Tetrametilbencidina
Brand: SIGMA

TMB substrate, chromogenic, ≥98% (TLC), powder

Sinónimos:

BM azul, Sure Blue TMB, Sustrato TMB, TMB, Transferencia de TMB Plus

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About This Item

Fórmula lineal:

[-C₆H₂(CH₃)₂-4-NH₂]₂

Número CAS:

54827-17-7

Peso molecular:

240.34

NACRES:

NA.83

PubChem Substance ID:

24900090

UNSPSC Code:

12352204

EC Number:

259-364-6

MDL number:

MFCD00007748

Beilstein/REAXYS Number:

2808541

Nombre del producto

3,3′,5,5′-Tetrametilbencidina, ≥98% (TLC)

InChI key

UAIUNKRWKOVEES-UHFFFAOYSA-N

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

SMILES string

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

assay

≥98% (TLC)

form

powder

mp

168-171 °C (lit.)

solubility

ethyl acetate: 50 mg/mL, clear to very slightly hazy

storage temp.

2-8°C

Quality Level

200

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Categorías relacionadas

Sustratos y enzimas de detección

Application

La 3,3′,5,5′-tetrametilbencidina (TMB) es un sustituto no carcinógeno (prueba de Ames negativa) de la bencina, adecuada como sustrato de la peroxidasa para procedimientos ELISA. Este sustrato rinde un producto final soluble que es de color azul claro y puede leerse espectrofotométricamente a 370 o 620-650 nm. La reacción de la TMB puede detenerse con H₂SO₄ 2 M (que produce un color amarillo) y leerse a 450 nm. Reactivo sensible y específico para la detección de sangre, análisis de la hemoglobina, análisis de peroxidasa.

3,3′,5,5′-Tetramethylbenzidine has been used:

as a supplement in Man-Rodosa-Sharpe (MRS) agar plate, to stains the lactobacilli colonies blue in the presence of O2
as a substrate solution for ELISA (enzyme-linked immunosorbent assay)
in myeloperoxidase activity assay to quantify neutrophil

Biochem/physiol Actions

3,3′,5,5′-Tetramethylbenzidine/TMB can be used as a chromogen to increase the progression of product obtained in peroxidase reaction. In food and environmental decontamination procedures, TMB can be used in in situ free available chlorine (FAC) monitoring of chlorite-based sanitizers.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

pictograms

GHS08,GHS07

signalword

Warning

hcodes

H302,H351,H413

pcodes

P202 - P264 - P273 - P280 - P301 + P312 - P308 + P313

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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Ferrotransferrin and antibody against the transferrin receptor as potential vehicles for drug delivery across the mammalian blood-brain barrier into the central nervous system

Methods in Neurosciences, 21(1), 93-117 (1994)

Lactobacilli isolated from vaginal vault of dairy and meat cows during progesteronic stage of estrous cycle

Rodriguez C, et al.

Anaerobe, 17(1), 15-18 (2011)

The CD4+ T cell-mediated IFN-gamma response to Helicobacter infection is essential for clearance and determines gastric cancer risk

Sayi A, et al.

Journal of immunology (Baltimore, Md. : 1950), 182(11), 7085-7101 (2009)

Lactobacillus species identification, H2O2 production, and antibiotic resistance and correlation with human clinical status.

A Felten et al.

Journal of clinical microbiology, 37(3), 729-733 (1999-02-13)

Lactobacilli recovered from the blood, cerebrospinal fluid, respiratory tract, and gut of 20 hospitalized immunocompromised septic patients were analyzed. Biochemical carbohydrate fermentation and total soluble cell protein profiles were used to identify the species. Hydrogen peroxide production was measured. Susceptibility

Tetramethylbenzidine method for monitoring the free available chlorine and microbicidal activity of chlorite-based sanitizers under organic-matter-rich environments

Yamaoka H, et al.

Letters in Applied Microbiology, 62(1), 47-54 (2016)

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Contenido relacionado

Product Information Sheet - T2885

Instructions