T3526

Anti-TUBA1B (alpha-tubulin ubiquitous) Antibody

Name: Anti-TUBA1B (alpha-tubulin ubiquitous) Antibody
Brand: SIGMA

rabbit polyclonal

Sinónimos:

Anti-K-ALPHA-1

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

MDL number:

MFCD00145921

Nombre del producto

Anti-Tubulin antibody produced in rabbit, whole antiserum

biological source

rabbit

conjugate

unconjugated

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

species reactivity

chicken, mouse

technique(s)

indirect immunofluorescence: 1:80 using cultured fibroblasts
western blot: 1:200 using chicken gizzard extract or mouse brain extract

UniProt accession no.

P68363

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Application

Anti-Tubulin antibody produced in rabbit has been used in immunoblotting and indirect immunofluorescence.

Biochem/physiol Actions

αβ-tubulin heterodimers helps in formation of microtubules. Tubulin dimers exhibits GTPase activity, assembly and disassembly properties. It facilitates the construction of the flagellar apparatus.

The product stains tubulin in cultured chicken fibroblasts or 3T3 mouse fibroblasts. Additional bands may be stained in immunoblotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Microtubules are composed of tubulin and several additional proteins designated microtubule associated proteins (MAPs). Tubulin is a heterodimer which consists of an α-chain and a β-chain, both of which have a molecular weight of approximately 55,000. Microtubules form an elaborate cytoplasmic network during interphase and are reorganized into the mitotic spindle during cell division.

Tubulin is an extremely conserved and negatively charged protein. It is expressed in all eukaryotic cells, but mainly in brains.

Immunogen

microtubule proteins from brains of 13 day old chicken embryos

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Clase de almacenamiento

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificados de análisis (COA)

Lot/Batch Number

0000508184

0000359483

0000237218

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Hong Zhu et al.

Experimental and therapeutic medicine, 15(3), 2643-2648 (2018-02-20)

The aim of the present study was to investigate the mechanism of action by which naringin reverses the resistance of ovarian cancer cells to cisplatin. Semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) and western blotting assays were used to detect

Microtubule proteins and their post-translational forms in the cerebrospinal fluid of patients with paraparesis associated with HTLV-I infection and in SH-SY5Y cells: an in vitro model of HTLV-I-induced disease

Maldonado H, et al.

Biological Research, 41(3), 239-259 (2008)

Tubulin and microtubule structure

Downing KH and Nogales E

Current Opinion in Cell Biology, 10(1), 16-22 (1998)

Loss of AND-34/BCAR3 expression in mice results in rupture of the adult lens.

Richard I Near et al.

Molecular vision, 15, 685-699 (2009-04-15)

AND-34/BCAR3 (Breast Cancer Anti-Estrogen Resistance 3) associates with the focal adhesion adaptor protein, p130CAS/BCAR1. Expression of AND-34 regulates epithelial cell growth pattern, motility, and growth factor dependence. We sought to establish the effects of the loss of AND-34 expression in

Tubulin and CRMP-2 complex is transported via Kinesin-1.

Toshihide Kimura et al.

Journal of neurochemistry, 93(6), 1371-1382 (2005-06-07)

The transport of tubulin and microtubules in a growing axon is essential for axonal growth and maintenance. However, the molecular mechanism underlying the linkage of tubulin and microtubules to motor proteins is not yet clear. Collapsin response mediator protein-2 (CRMP-2)

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Data Sheet - T3526

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