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Merck

T4069

JC-1

≥97% purity, solid

Sinónimos:

5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide, 5,5′,6,6′-Tetrachloro-1,1′,3,3′-tetraethylbenzimidazolocarbocyanine iodide, CBIC2(3)

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About This Item

Fórmula empírica (notación de Hill):
C25H27Cl4IN4
Número CAS:
3520-43-2
Peso molecular:
652.23
UNSPSC Code:
12171500
PubChem Substance ID:
24900193
NACRES:
NA.47
MDL number:
MFCD00188475

Nombre del producto

JC-1, powder or solid (Crystals)

SMILES string

[I-].CCN1C(=C\C=C\c2n(CC)c3cc(Cl)c(Cl)cc3[n+]2CC)/N(CC)c4cc(Cl)c(Cl)cc14

InChI

1S/C25H27Cl4N4.HI/c1-5-30-20-12-16(26)17(27)13-21(20)31(6-2)24(30)10-9-11-25-32(7-3)22-14-18(28)19(29)15-23(22)33(25)8-4;/h9-15H,5-8H2,1-4H3;1H/q+1;/p-1

InChI key

FYNNIUVBDKICAX-UHFFFAOYSA-M

assay

≥97%

form

powder or solid (Crystals)

technique(s)

titration: suitable

color

faint red to very dark red

ε (extinction coefficient)

≥175000 at 511-517 nm in methanol

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

100

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Application

A dual-emission potential-sensitive probe that can be used to measure mitochondrial membrane potential. JC-1 is a green-fluorescent (λex 520 nm) monomer at low membrane potential. At higher potentials, JC-1 forms red-fluorescent (λem 596 nm ) "J-aggregates," which exhibit broad excitation and very narrow emission spectra. The ratio of red to green fluorescence of JC-1 is dependent only on membrane potential, and not influenced by mitochondrial size, shape, or density.
The cyanine dye JC-1 has been used to determine mitochondrial transmembrane potential.

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
MKCZ5582
MKCX9305
MKCX2204
MKCW5291
MKCT7568

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Junctophilin 3 expresses in pancreatic beta cells and is required for glucose-stimulated insulin secretion
Li L
Cell Death & Disease (2016)
Shumin Qin et al.
Frontiers in pharmacology, 10, 894-894 (2019-09-03)
Alcohol consumption affects gastric mucosa by multiple and complex mechanisms depending either by direct contact of ethanol or by indirect biological damage induced by its metabolite acetaldehyde. The present study aims at further investigating the mechanism of ethanol-induced gastric mucosa
JC-1: alternative excitation wavelengths facilitate mitochondrial membrane potential cytometry
A Perelman
Cell Death & Disease (2012)
Xianbin Cao et al.
Cellular signalling, 62, 109337-109337 (2019-06-08)
Radiation-induced tumor cells death is the theoretical basis of tumor radiotherapy. Death signaling disorder is the most important factor for radioresistance. However, the signaling pathway(s) leading to radiation-triggered cell death is (are) still not completely known. To better understand the
Sulfated polysaccharide-protein complex sensitizes doxorubicin-induced apoptosis of breast cancer cells in vitro and in vivo
Jie Wang
Experimental and Therapeutic Medicine (2016)
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