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Merck

U1500

Urease from Canavalia ensiformis (Jack bean)

Type III, powder, 15,000-50,000 units/g solid

Sinónimos:

Jack bean urease, Urea amidohydrolase

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Número CAS:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-656-0
NACRES:
NA.54
MDL number:
Número CE:
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Nombre del producto

Urease from Canavalia ensiformis (Jack bean), Type III, powder, 15,000-50,000 units/g solid

biological source

Canavalia ensiformis

type

Type III

form

powder

specific activity

15,000-50,000 units/g solid

mol wt

~544620 Da

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

Quality Level

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Biochem/physiol Actions

Urease from Canavalia ensiformis functions as an insecticide by the release of a toxic small peptide cleaved by proteases found in the gut of insects.

General description

Subunit molecular weight: ~90,770
Composed of six subunits with total molecular weight: ~544,620
Urease is a cytosolic enzyme that catalyzes the hydrolysis of urea to ammonia and carbon dioxide, with initial conversion to carbamate, and the carbamate breaks down further, on hydrolysis, into ammonia and to carbonic acid / carbonate. Urease is a metalloenzyme that contains nickel as a co-factor. Urease occurs naturally in plants, fungi, and certain bacteria. James B. Sumner first isolated urease from jack bean in 1926.

Other Notes

One micromolar unit will liberate 1.0 μmole of NH3 from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)

pictograms

Health hazardExclamation mark

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Clase de almacenamiento

11 - Combustible Solids

wgk

WGK 1


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Han-Ho Kim et al.
Journal of microbiology and biotechnology, 31(12), 1684-1691 (2021-12-29)
In this study, the physicochemical changes related to fishy smell were determined by storing high hydrostatic pressure (HHP)-treated mackerel (Scomber japonicus) meat in a refrigerator for 20 days. The inhibition of crude urease activity from Vibrio parahaemolyticus using HHP treatment
Karl Norris et al.
Marine drugs, 18(6) (2020-06-06)
Whey protein isolate (WPI) is a by-product from the production of cheese and Greek yoghurt comprising β-lactoglobulin (β-lg) (75%). Hydrogels can be produced from WPI solutions through heating; hydrogels can be sterilized by autoclaving. WPI hydrogels have shown cytocompatibility and
Jungyeon Kim et al.
PloS one, 15(3), e0230072-e0230072 (2020-03-18)
In the urinary metabolomics for finding biomarkers in urine, owing to high concentrations of urea, for chromatography-based metabolomic analysis, urea needed to be degraded by urease. This urease pretreatment has been the key step of sample preparation for standard urinary
Fernanda Stanisçuaski et al.
Journal of insect physiology, 55(3), 255-263 (2009-01-06)
Ureases are metalloenzymes that are widespread among plants, fungi and bacteria. Urease isoforms (jack bean urease-JBU and canatoxin) from Canavalia ensiformis seeds are toxic to insects and fungi, suggesting a role in plant defense. The entomotoxic effect is due to
Sun-Il Kim et al.
Microorganisms, 10(1) (2022-01-22)
Ammonia from livestock manure reacts with chemical components discharged from various emission sources to produce airborne particulate matter. This study aimed to investigate a novel effective microbial agent to suppress ammonia gas emitted from manure. Both isolated L12I and 12III

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