W0138
Western Blocker™ Solution
for HRP detection systems
Sinónimos:
Western blot blocking solution, blocking buffer
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About This Item
NACRES:
NA.25
UNSPSC Code:
41105329
Nombre del producto
Western Blocker™ Solution, for HRP detection systems
shelf life
≥2 yr
storage temp.
2-8°C
Quality Level
Application
Western Blocker Solution has been optimized for detection systems using horseradish peroxidase (HRP) conjugates; it is also compatible with alkaline phosphatase based systems.
Western Blocker Solution has been used for blocking of western blot membranes. It has also been used for dilution of antibodies in western blot.
Western Blocker Solution has been used for blocking of western blot membranes. It has also been used for dilution of antibodies in western blot.
Features and Benefits
- Does not inhibit weak HRP signals, as many milk-based blockers do.
- Can be used both as a blocking component and as a probing component.
- Supplied ready-to-use; no preparative step is required.
General description
Western Blocker Solution is used to block both PVDF and nitrocellulose membranes after proteins have been transferred onto them through dot blotting or Western blotting; blocking is performed to reduce or eliminate nonspecific binding of primary or secondary antibodies.
Legal Information
Western Blocker is a trademark of Sigma-Aldrich Co. LLC
Clase de almacenamiento
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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