1.05438
Sabouraud Dextrose Agar
GranuCult® prime, Dextrose 4 %, EP 5.6, USP 29, JP 4.05, granular, for yeasts, for molds, pack of 500 g and 5 kg
Synonym(s):
Fungus culture medium acc. to SABOURAUD, SABOURAUD 4% dextrose agar
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About This Item
NACRES:
NA.74
UNSPSC Code:
41106213
Quality Level
agency
EP 5.6, JP 4.05, USP 29
form
granular
composition
Dextrose, 4%
manufacturer/tradename
GranuCult® prime
packaging
pack of 500 g and 5 kg
technique(s)
microbiological culture: suitable
pH
5.6 (25 °C, 65 g/L in H2O, after autoclaving)
solubility
65 g/L
bulk density
680 kg/m3
application(s)
bioburden testing
industrial qc
microbiology
pharmaceutical
storage temp.
15-25°C
suitability
molds, yeasts
General description
Sabouraud 4% Dextrose Agar is a complex, granulated, dehydrated culture medium for cultivation and isolation of yeasts and molds. It also serves as the absence test for Candida albicans. The high concentration of dextrose and the low pH promotes the growth, the formation of spores (Conidia and Sporangia), and the formation of pigments of yeasts and molds. Additionally, it inhibits the growth of bacteria.
Application
Sabouraud 4% Dextrose Agar is designed for the determination of the total count of yeasts and molds in non-sterile products.
Analysis Note
Appearance (clearness): clear
Appearance (colour): yellowish-brown
pH-value (25 °C): 5.4 - 5.8
Typical composition (g/litre): Peptone from casein5.0; Peptone from meat 5.0; D(+)Glucose 40.0; Agar-agar 15.0.
Growth (Trichophyton mentagrophytes ATCC 18748): fair to very good
Growth (Trichophyton rubrum ATCC 28188): fair to good
Growth (Trichophyton ajelloi ATCC 28454): fair to good
Growth (Microsporum gallinae ATCC 12108): fair to very good
Growth (Microsporum canis ATCC 36299): good to very good
Growth (Geotrichum candidum DSM 1240): good to very good
Growth (Penicillium commune ATCC 10428): good to very good
Incubation: 7 days; 28°C
Growth promotion test in accordance with the harmonised method of EP, USP and JP.
Inoculum on reference medium (Candida albicans ATCC 10231 (WDCM 00054)): 10 - 100
Inoculum on reference medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): 10 - 100
Colony count (Candida albicans ATCC 10231 (WDCM 00054)):
Colony count (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)):
Recovery on test medium (Candida albicans ATCC 10231 (WDCM 00054)): ≥ 70 %
Recovery on test medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): ≥ 50 %
Incubation: C. albicans and A. brasiliensis up to 5 daysat 20-25°C.
The information above is current at this time of publication and is subject to change without notice (except for customers holding a change control agreement with our company). The information/format can be altered at any time and inany way if internal company matters or Standard Regulationsdo say so.
Appearance (colour): yellowish-brown
pH-value (25 °C): 5.4 - 5.8
Typical composition (g/litre): Peptone from casein5.0; Peptone from meat 5.0; D(+)Glucose 40.0; Agar-agar 15.0.
Growth (Trichophyton mentagrophytes ATCC 18748): fair to very good
Growth (Trichophyton rubrum ATCC 28188): fair to good
Growth (Trichophyton ajelloi ATCC 28454): fair to good
Growth (Microsporum gallinae ATCC 12108): fair to very good
Growth (Microsporum canis ATCC 36299): good to very good
Growth (Geotrichum candidum DSM 1240): good to very good
Growth (Penicillium commune ATCC 10428): good to very good
Incubation: 7 days; 28°C
Growth promotion test in accordance with the harmonised method of EP, USP and JP.
Inoculum on reference medium (Candida albicans ATCC 10231 (WDCM 00054)): 10 - 100
Inoculum on reference medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): 10 - 100
Colony count (Candida albicans ATCC 10231 (WDCM 00054)):
Colony count (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)):
Recovery on test medium (Candida albicans ATCC 10231 (WDCM 00054)): ≥ 70 %
Recovery on test medium (Aspergillus brasiliensis (formerly A. niger) ATCC 16404 (WDCM 00053)): ≥ 50 %
Incubation: C. albicans and A. brasiliensis up to 5 daysat 20-25°C.
The information above is current at this time of publication and is subject to change without notice (except for customers holding a change control agreement with our company). The information/format can be altered at any time and inany way if internal company matters or Standard Regulationsdo say so.
Legal Information
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered® culture media, depending on your needs. The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
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Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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