324708
Asp-N Protease
Cleaves N-terminal to aspartic acid residues, suitable for Mass Spectrometry, from Pseudomonas fragi mutant strain
Synonym(s):
Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi
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About This Item
CAS Number:
MDL number:
NACRES:
NA.54
UNSPSC Code:
12352202
eCl@ss:
32160410
Specific activity:
≥20,000 units/mg protein
Assay:
≥90% (SDS-PAGE)
Product Name
Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi,
grade
Proteomics Grade
Quality Level
assay
≥90% (SDS-PAGE)
form
lyophilized solid
specific activity
≥20,000 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
color
white
solubility
water: soluble
shipped in
ambient
storage temp.
2-8°C
General description
Native endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
Physical form
Lyophilized from 10 mM Tris-HCl, pH 7.5.
Preparation Note
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
Other Notes
Drapeau, G.R. 1980. J. Biol. Chem.255, 839.
One unit is defined as the amount of enzyme that will release a sufficient amount of azo dye from AZOCOLL Substrate (Cat. No. 194932) to produce an increase in absorbance at 520 nm of 0.001 per min at 37°C, pH 7.5.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Harmful (C)
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Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 1
Certificates of Analysis (COA)
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