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ABC962

Anti-CDC45

Name: Anti-CDC45
Brand: MM

from rabbit, purified by affinity chromatography

Synonym(s):

Cell division control protein 45 homolog, PORC-PI-1

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

polyclonal

Application:

ICC, WB

Citations:

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Properties

biological source

rabbit

Quality Level

100

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human

species reactivity (predicted by homology)

hamster (based on high sequence homology), rat (based on high sequence homology)

technique(s)

immunocytochemistry: suitable, western blot: suitable

NCBI accession no.

NP_003495

UniProt accession no.

O75419

shipped in

ambient

target post-translational modification

unmodified

Gene Information

hamster ... Cdc45(100689362)
human ... CDC45(8318)
mouse ... Cdc45(12544)
rat ... Cdc45(287961)

Description

General description

Cell division control protein 45 homolog (UniProt O75419; also known as PORC-PI-1) is encoded by the CDC45 (also known as CDC45L, CDC45L2, UNQ374/PRO710) gene (Gene ID 8318) in human. Recruitment of replication initiation proteins at the G1/S transition enables origin DNA unwinding and loading of DNA polymerases. CDC45 is a replication initiation protein that plays an essential role in the establishment and progression of the replication fork. Cdc45 associates with the heterohexameric Mcm2-7 helicase and the GINS complex (go-ichi-nii-san, Japanese for 5-1-2-3, for the subunits Sld5, Psf1, Psf2 and Pfs3) to form the CMG (Cdc45-Mcm2-7-GINS) complex. CMG represents the eukaryotic replicative DNA helicase that unwinds origins of replication and drives the replication fork. CDC45 is also reported to interact with several other replication proteins, including DNA helicase B (DHB), Mcm10, Orc2, TopPB1, and DNA polymerases alpha, delta and epsilon.

~75 kDa observed. 65.57/60.25/68.77 kDa (human isoform 1/2/3), 65.38 kDa (mouse) calculated. Uncharacterized bands may be observed in some lysate(s).

Immunogen

GST-tagged recombinant human CDC45 C-terminal fragment.

Application

Detect CDC45 using this rabbit polyclonal Anti-CDC45 Antibody, Cat. No. ABC962, validated for use in Immunocytochemistry and Western Blotting.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected CDC45 in L929 murine fibroblasts.

Biochem/physiol Actions

This polyclonal antibody detects CDC45 C-terminal region highly conserved among human, mouse, rat, and hamster species.

Analysis Note

Evaluated by Western Blotting in L929 fibroblast lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected CDC45 in 10 µg of L929 murine fibroblast lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c