Anti-CD244/2B4 Antibody, clone C1.7

Natural killer cell receptor 2B4 (UniProt: Q9BZW8; also known as CD244, NK cell activation-inducing ligand (NAIL), NK cell type I receptor protein 2B4 (NKR2B4; h2B4), SLAM family member 4 (SLAMF4), Signaling lymphocytic activation molecule 4) is encoded by the CD244 (also known as 2B4) gene (Gene ID: 51744) in human. CD244 (2B4) is a SLAM family receptor broadly expressed on NK cells, subsets of CD8+ αβ and γδ T cells, dendritic cells, and myeloid-derived suppressor cells (MDSCs), where it binds CD48 and signals via four ITSM motifs that recruit SH2 adaptors to drive activating (SAP) or inhibitory (SHP1/SHP2/SHIP-1; context-dependent EAT-2) outcomes; the net signal is governed by receptor density and adaptor availability, with high CD244 and/or limited SAP favoring inhibition. In the tumor microenvironment, CD244 signaling is predominantly inhibitory—promoting NK and CD8+ T-cell exhaustion and dampening cytokine production, while in DCs and Gr-MDSCs it aligns with increased immunosuppressive function—mechanisms often dependent on CD244–CD48 interactions with tumor-associated immune cells. These insights support therapeutic CD244 blockade to reinvigorate exhausted effectors, enhance proinflammatory DC activity, and diminish MDSC-mediated suppression; supportive preclinical data, the grossly normal phenotype of CD244−/− mice, and evidence that leukemia cells can depend on CD244 signaling, collectively underscore CD244 as a promising immuno-oncology target.

Cultured human NK cells.

Quality Control Testing

Evaluated by Flow Cytometry in human Peripheral Blood Mononuclear Cells (PBMC).
Flow Cytometry Analysis: 1.0 µg from a representative lot detected CD244/2B4 in one million human Peripheral Blood Mononuclear Cells (PBMC).
Inhibition:  A representative lor of this antibody inhibited non-MHC-restricted cytotoxicity mediated by resting NK cells and rlL-2- cultured T cells. (Valiante, N.M., et al. (1993). J Exp Med. 178(4):1397-406).
Western Blotting Analysis: A representative lot detected CD244/2B4 in Western Blotting application (Valiante, N.M., et al. (1993). J Exp Med. 178(4):1397-406).
Flow Cytometry Analysis: A representative lot detected CD244/2B4 in Flow Cytometry application (Valiante, N.M., et al. (1993). J Exp Med. 178(4):1397-406).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone C1.7 is a mouse monoclonal antibody that detects CD244/2B4. It targets an epitope from the extracellular domain.

Purified mouse monoclonal antibody IgG1κ in PBS without azide.

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

1. Valiante, N.M., et al. (1993). J Exp Med. 178(4):1397-406.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.