M1533
N,N′-Methylenebisacrylamide solution
suitable for electrophoresis, 2% in H2O
Synonym(s):
BIS
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About This Item
Linear Formula:
(CH2=CHCONH)2CH2
CAS Number:
Molecular Weight:
154.17
UNSPSC Code:
41105319
NACRES:
NB.22
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
1706297
sterility
0.2 μm filtered
Quality Level
form
liquid
concentration
2% in H2O
technique(s)
electrophoresis: suitable
storage temp.
2-8°C
SMILES string
C=CC(=O)NCNC(=O)C=C
InChI
1S/C7H10N2O2/c1-3-6(10)8-5-9-7(11)4-2/h3-4H,1-2,5H2,(H,8,10)(H,9,11)
InChI key
ZIUHHBKFKCYYJD-UHFFFAOYSA-N
General description
N,N′-Methylenebisacrylamide solution is a cross-linking agent used in polyacrylamide gel preparation.
Application
N,N′-Methylenebisacrylamide solution has been used as a component of hydrogel precursor solution and magnified analysis of the proteome (MAP) gel solution during hydrogel fabrication. It has also been used as a component of re-embedding solution during re-embedding of expanded hydrogels.
To be mixed with acrylamide for making polyacrylamide gels for use in protein and nucleic acid electrophoresis.
Preparation Note
Prepared from electrophoresis grade bis-acrylamide in ultra-pure water.
signalword
Danger
hcodes
Hazard Classifications
Carc. 1B - Muta. 1B - STOT RE 2 Oral
target_organs
Peripheral nervous system
Storage Class
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Faceshields, Gloves, multi-purpose combination respirator cartridge (US)
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Related Content
Datasheet
Chapter 15 - Techniques for Protein Analysis
Barh D and Azevedo V
Omics : A Journal of Integrative Biology null
A J Aho et al.
British journal of anaesthesia, 115(2), 258-266 (2015-07-04)
Concomitantly recorded Bispectral Index® (BIS) and Entropy™ values sometimes show discordant trends during general anaesthesia. Previously, no attempt had been made to discover which EEG characteristics cause discrepancies between BIS and Entropy. We compared BIS and Entropy values, and analysed
Alice E Stanton et al.
Acta biomaterialia, 96, 310-320 (2019-07-01)
Extracellular matrix (ECM) is comprised of different types of proteins, which change in composition and ratios during morphogenesis and disease progression. ECM proteins provide cell adhesion and impart mechanical cues to the cells. Increasing substrate stiffness has been shown to