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RAB0143

Human Dkk-1 ELISA Kit

Name: Human Dkk-1 ELISA Kit
Brand: SIGMA

for serum, plasma, cell culture supernatant and urine

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About This Item

NACRES:

NA.84

UNSPSC Code:

41116158

Gene information:

human ... DKK1(22943)

Input:

sample type serum
sample type urine
sample type plasma
sample type cell culture supernatant(s)

Species reactivity:

human

Storage temp.:

−20°C

Shipped in:

wet ice

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Properties

species reactivity

human

packaging

kit of 96 wells (12 strips x 8 wells)

technique(s)

ELISA: suitable, capture ELISA: suitable

input

sample type serum
sample type urine
sample type plasma
sample type cell culture supernatant(s)

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 100 pg/mL
standard curve range: 122.9-30000 pg/mL

detection method

colorimetric

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... DKK1(22943)

Description

General description

The human Dkk-1 (hDkk-1) gene, a transcriptional target of the p53 tumor suppressor, encodes a powerful inhibitor of the Wnt signaling pathway and regulates the spatial patterning/morphogenesis of the mammalian central nervous system.The Dkk-1 gene, also called Dickkopf-1 , is a prototype member of a recently identified gene family that encodes secreted glycoproteins that control cell fate and neural patterning during embryonic development. This gene is located on human chromosome 10q.

Immunogen

Recombinant Human DKK1

Application

For research use only. Not for use in diagnostic procedures.
Please refer to the attached General ELISA KIT Procedure (sandwich, competitive & Indirect ELISA)

Biochem/physiol Actions

Evidence strongly indicates that Dkk-1 protein functions as a potent inhibitor of the Wnt signaling pathway to enable appropriate positioning and development of the embryonic brain and other organ structures. hDkk-1 protein also has a pro-apoptotic function.

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

Product No.

Description

SDS & Pricing

RABELADB
ELISA 5X Assay/Sample Diluent Buffer B (Item E1)
SDS

RABELADD
ELISA 5X Assay/Sample Diluent Buffer D (Item K)
SDS

RABSTOP3
ELISA Stop Solution (Item I)
SDS

RABTMB3
ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)
SDS

RABWASH4
20X Wash Buffer (Item B)
SDS

pictograms

GHS05

signalword

Warning

hcodes

H290

pcodes

P234 - P390

Hazard Classifications

Met. Corr. 1

Storage Class

10 - Combustible liquids

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Certificates of Analysis (COA)

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