Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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96-Well Plate
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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The choice of cell line, primary cells, or multicellular developmental model is critical for live cell analysis. Many cellular model systems have particular growth characteristics or behaviors that make them unsuitable for live cell analysis, while others seem ideally suited and can tolerate the sometimes less than ideal “living” conditions often necessary for live cell analysis, particularly for extended time analyses.
Primary cell lines, being extracted directly from tissue, are often an attractive choice in live cell analysis because they are presumably closer to their natural origins and thus may be more relevant in predictive cell culture. There are a large number of primary cultures that can be extracted and maintained from tissues; however, most have similar limitations in growth rate, passaging, and maintaining in vivo-like characteristics. The more passaging that is done, the less a primary cell culture is truly “primary”. They are often less controllable than cell lines so it is widely acknowledged that when using primary cultures, know your source populations well, control their culture environment carefully, and validate their phenotypes periodically (such as with antibody staining patterns and karyotyping).
Example of Primary Cultures Useful for Live Cell Analysis
With the plethora of cell lines available today the choice for investigators is quite large. Many cell lines today have become the “go to” cell line for examining particular cellular events such as apoptosis, or cellular transport. Researchers should certainly consult the growing literature available using live cell analysis and use it for guidance. Instrumentation improvements continue to expand our ability to control and define both the extrinsic and intrinsic factors necessary for even the most sensitive cell cultures.
Example Cell Lines Useful for Live Cell Analysis
Cell Line
Cell Type
Origin
Species
Sample Application/ Uses
B-16
Spindle
Melanoma
Mouse
Cancer, melanoma studies
BAE-1
Endothelial
Aorta endothelium
Bovine
Endothelial barriers
BHK-21
Fibroblast
Kidney
Hamster
Cytoskeletal dynamics
CACO-2
Epithelial
Colon
Human
Transport studies, cell junctions
CHO-K1
Epithelial
Ovary
Hamster
EGFR mutations, Drug transport
HEK293
Epithelial / endothelial
Kidney
Human
RNAi, channels, metabolism, viral infections
HELA
Epithelial
Cervix
Human
Cellular processes, apoptosis, cancer research
Hep G2
Epithelial
Liver
Human
Liver metabolism, cell polarization
L929
Epithelial
Connective tissue
Mouse
Apoptosis, cytokine response
LT2
Epithelial
Pancreas
Human
Cancer, anchorage dependence
MCF-7
Epithelial
Mammary
Human
Apoptosis, cancer, estrogen receptor research
MDCK
Epithelial
Kidney
Dog
Cytokeratin, Cell Junction studies
NIH-3T3
Fibroblast
Embryo
Mouse
Fibroblast metabolism, cell division, cell cycle
PC-12
Aggregate
Adrenal Gland
Rat
Nerve Growth Factor response, neurite formation
PtK2
Epithelial
Kidney
Rat Kangaroo
Mitosis, cytoskeletal studies, intracellular transport
Yeast, bacterial, and fungal cultures are also being studied using in live cell platforms for a variety of research topics including host pathogen interactions, infection, toxicology/drug response, chemotaxis/migration, microenvironment dynamics, microbiome, and bacterial or yeast single cell analysis.
Application Note Download:
Read about live cell analysis of host pathogen interactions in long term cultures.
Since the advent of molecular genetics, certain multicellular models have emerged as extremely useful systems for the study of development, genetic manipulations, toxicity, signaling, neural processing, liver and digestive tract function, and behavior.
Examples of Animal Models Used in Live Cell Analysis
Species
Characteristics
Sample Application/ Uses
Zebrafish
Defined genetics, optically clear embryo
Organ development, Neural connectivity, physiology, oxidative stress
Drosophila
Defined, manipulatable genetics, short lifecycle
Genetics, Organ development, Neural connectivity
C. Elegans
Defined genetics and limited cell types
Genetics, early development, cellular interactions