122643 ProteoExtract® Albumin/IgG Removal Kit, Maxi

122643
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Overview

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122643-1KIT
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      Description
      OverviewHigh capacity, reusable columns specifically designed for the rapid and efficient removal of >80% of albumin (column 1 containing albumin-affinity resin) and IgG (column 2 containing immobilized protein A) from body fluid samples, either on an LC-instrument or manually, attached to a syringe. Removal of these high abundance proteins allows enhanced detection of low abundance proteins.
      Catalogue Number122643
      Brand Family Calbiochem®
      Materials Required but Not Delivered Micropipettes and tips, 10 µl, 200 µl and 1 ml size
      Syringes with different volumes for manual usage.
      High quality water (resistance > 18 mΩ)
      Sample collection tubes e.g. 15ml centrifuge tubes
      References
      ReferencesBoyle, M. D. P. and K. J. Reis., 1987. Biotechnology 5, 697.
      Product Information
      Form20 Albumin-containing samples (60-180 μl each) and 10 IgG-containing samples (60-180 μl each)
      FormatAffinity column
      Kit containsTwo Albumin Removal Columns, one IgG Removal Column, 10X Albumin/IgG Binding Buffer, Albumin Elution Buffer, IgG Elution Buffer, and a user protocol.
      Applications
      Biological Information
      Sample TypeSerum, plasma, CSF
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Intended useThe ProteoExtract® Albumin/IgG Removal Kit, Maxi provides a convenient, reproducible and highly specific method to remove serum albumin and immunoglobulins from body fluid samples. The kit provides columns containing either a novel albumin-specific affinity resin or a proprietary immobilized protein A polymeric resin. These columns allow for the removal of more than 80% of both serum albumin and immunoglobulins from human body fluid samples when used according to the instructions provided in this manual. The column hardware is designed to allow for both manual and LC-instrument controlled usage. When used manually, the columns are operated as syringe-tip filters, when used with LC-instrument control, the columns can be connected to any standard HPLC/FPLC instrument via a 10/32" port using the provided adapters. For guidelines of column connections and operation, please refer to section 3 of this manual.
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage +2°C to +8°C
      Storage ConditionsUpon arrival store entire contents of the kit at 4°C. Avoid freezing of the ProteoExtract® Blue and ProteoExtract® Protein A columns! Freezing thawing will destroy the affinity resin and the columns no longer function.
      Protect from Light Protect from light
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Kit containsTwo Albumin Removal Columns, one IgG Removal Column, 10X Albumin/IgG Binding Buffer, Albumin Elution Buffer, IgG Elution Buffer, and a user protocol.
      Specifications

      Documentation

      References

      Reference overview
      Boyle, M. D. P. and K. J. Reis., 1987. Biotechnology 5, 697.

      User Protocols

      Title
      User Protocol