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Developed in 1983, the ELISpot assay represents the convergence of plate-based Enzyme Linked Immunosorbent Assays (ELISAs) with membrane-based Western blotting technologies, permitting detection of secreted analytes at the single cell level. Originally conceived for the enumeration of B cells secreting antigen-specific antibodies, the ELISpot has been adapted for many tasks, the most prominent being the quantification of antigen-specific cytokine responses (See image below). Since its initial development, the ELISpot assay has been commonly applied to inflammation and cytokine responses for a variety of diseases from infections to cancer.