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Choose the for rapid isolation of total DNA from a variety of solid tissues or the PureGenome™ On-Spot Tissue DNA Kit for extracting DNA from targeted tissue sections (includes formalin fixed paraffin-embedded (FFPE) tissues). Furthermore, you can use these kits to process samples of virtually any biological liquids, such as whole blood, plasma, serum or cells from culture.
High-Quality DNA Isolation from FFPE Sample
PureGenome™ On-Spot Tissue DNA Kit was used to isolate total DNA from FFPE tissue sections (normal human liver). An area of ~ 100 mm2 was selected on two human liver FFPE tissue slides. Quantitative PCR was used to detect either a mitochondrial (ND6; purple) or a nuclear-encoded gene (BECN1; blue) from the DNA isolated using the PureGenome™ On-Spot Tissue DNA Kit. Input DNA was 2, 0.2 and 0.02 ng (curves labeled 1, 2 and 3, respectively).