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Merck

04-142

Anti-IRF4 Antibody, clone 3E4

clone 3E4, from rat

Synonym(s):

Interferon regulatory factor 4, IRF-4, Lymphocyte-specific interferon regulatory factor, LSIRF, NF-EM5, PU.1 interaction partner, Transcriptional activator PIP

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

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Product Name

Anti-IRF4 Antibody, clone 3E4, clone 3E4, from rat

biological source

rat

antibody form

purified antibody

antibody product type

primary antibodies

clone

3E4, monoclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology)

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_038702

UniProt accession no.

Q64287

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... IRF4(3662)
mouse ... Irf4(16364)

Physical form

Protein G Purified
Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified

Analysis Note

Evaluated by Western Blotting in Ramos cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected IRF4 in 10 µg of Ramos cell lysate.

Application

Anti-IRF4 Antibody, clone 3E4 is a highly specific rat monoclonal antibody, that targets IRF4 & has been tested in western blotting, IHC & Flow Cytometry.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected IRF4 in positive human tonsil tissues.

Flow Cytometry Analysis: A representative lot from an independent laboratory detected IRF4 in wildtype and IRF4 knockout mice (Zheng, Y., et al. (2009). Nature. 458(7236):351-356.).
Research Category
Inflammation & Immunology
Research Sub Category
Transcription Factors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Interferon regulatory factor 4, also known as IRF4, belongs to the IRF family. IRF4 encodes a 450 amino acid polypeptide with a predicted molecular weight of 51.6 kDa (Grossman 1996). The IRF4 gene is expressed in the spleen, lymphocytes and melanocytes, and is induced in to T cells after cross linking with the T cell receptor. IRF4 is a transcriptional activator that binds to the interferon stimulated response element (ISRE) of the MHC class I promoter. IRF4 is essential for mature T and B lymphocyte function. IRF4 interacts with MYD88 to prevent the interaction of IRF5, resulting in negative regulation of Toll Like Receptor (TLR) signaling. High levels of IRF4 have been associated with Epstein Barr Virus (EBV) and may be critical to EBV transformation. Further oncogenic potential has been associated with the involvement of IRF4 with hair color and pigmentation of the skin, which may cause multiple myeloma.
~51 kDa observed

Immunogen

GST-tagged recombinant protein corresponding to the C-terminus of mouse IRF4.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ye Zheng et al.
Nature, 458(7236), 351-356 (2009-02-03)
In the course of infection or autoimmunity, particular transcription factors orchestrate the differentiation of T(H)1, T(H)2 or T(H)17 effector cells, the responses of which are limited by a distinct lineage of suppressive regulatory T cells (T(reg)). T(reg) cell differentiation and

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