04-1540
Anti-PICH Antibody, clone 142-26-3
clone 142-26-3, from mouse
Synonym(s):
excision repair cross-complementing rodent repair deficiency, complementation group 6-like, excision repair cross-complementing rodent repair deficiency complementation group 6 - like, Tumor antigen BJ-HCC-15, PLK1-interacting checkpoint helicase, ATP-de
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-PICH Antibody, clone 142-26-3, clone 142-26-3, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
142-26-3, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ERCC6L(54821)
Analysis Note
Evaluated by Western Blot in HeLa-S3 cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Plk1-interacting checkpoint helicase on 10 µg of HeLa-S3 cell lysate.
Western Blot Analysis: 0.1 µg/mL of this antibody detected Plk1-interacting checkpoint helicase on 10 µg of HeLa-S3 cell lysate.
Control
HeLa-S3 cell lysate
HeLa-S3 cell lysate
Application
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP.
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC.
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in IC.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Use Anti-PICCH Antibody, clone 142-26-3 (mouse monoclonal antibody) validated in WB, IP, ICC to detect PICCH also known as Tumor antigen BJ-HCC-15, PLK1-interacting checkpoint helicase, ATP-dependent helicase ERCC6-like.
Biochem/physiol Actions
This antibody recognizes Plk1-interacting checkpoint helicase.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Plk1-interacting checkpoint helicase (PICH ) is an essential component of the spindle assembly checkpoint (Baumann, C., et al. (2007). Cell 128:101–114). This protein is an ATPase family SNF2 member, and serves as a binding partner and substrate for Plk1. When PICH is phosphorylated, it retrieves Plk1 which serves to mediate the localization of PICH. As a DNA helicase, PICH gathers at kinetochores and centromeres during prometaphase, acting as a necessary part of the spindle assembly checkpoint by recruiting MAD2 and mediating chromatin centromeric tension. Depleted PICH results in the cancellation of the spindle checkpoint causing large scale missegregation of the chromosome.
~160 kDa observed
Immunogen
Epitope: Unknown
Histidine-tagged recombinant protein corresponding to human Plk1-interacting checkpoint helicase.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Stefano Santaguida et al.
Developmental cell, 41(6), 638-651 (2017-06-21)
Aneuploidy, a state of karyotype imbalance, is a hallmark of cancer. Changes in chromosome copy number have been proposed to drive disease by modulating the dosage of cancer driver genes and by promoting cancer genome evolution. Given the potential of
Christoph Baumann et al.
Cell, 128(1), 101-114 (2007-01-16)
We identify PICH (Plk1-interacting checkpoint "helicase"), a member of the SNF2 ATPase family, as an interaction partner and substrate of Plk1. Following phosphorylation of PICH on the Cdk1 site T1063, Plk1 is recruited to PICH and controls its localization. Starting
Lotte P Watts et al.
eLife, 9 (2020-11-04)
Human cells lacking RIF1 are highly sensitive to replication inhibitors, but the reasons for this sensitivity have been enigmatic. Here, we show that RIF1 must be present both during replication stress and in the ensuing recovery period to promote cell
Christian F Nielsen et al.
Cell cycle (Georgetown, Tex.), 15(20), 2704-2711 (2016-08-28)
PICH is an SNF2-family DNA translocase that appears to play a role specifically in mitosis. Characterization of PICH in human cells led to the initial discovery of "ultra-fine DNA bridges" (UFBs) that connect the 2 segregating DNA masses in the
Federica Schiavoni et al.
Nature communications, 13(1), 1731-1731 (2022-04-03)
Aneuploidy results in decreased cellular fitness in many species and model systems. However, aneuploidy is commonly found in cancer cells and often correlates with aggressive growth, suggesting that the impact of aneuploidy on cellular fitness is context dependent. The BRG1
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 04-1540 | 04053252411298 |
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