04-1549

Anti-SAP130 Antibody, clone 1YO 1B10

Name: Anti-SAP130 Antibody, clone 1YO 1B10
Brand: MM

ascites fluid, clone 1YO 1B10, from mouse

Synonym(s):

130 kDa Sin3-associated polypeptide, Sin3-associated polypeptide p130, Sin3A-associated protein, 130kDa, histone deacetylase complex subunit SAP130, mSin3A-associated protein 130

Spliceosome-associated protein 130, Pre-mRNA-splicing factor SF3b

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

1YO 1B10, monoclonal

species reactivity

mouse, rat, human

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_598714.1

UniProt accession no.

Q921M3

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... SAP130(79595)

General description

SAP130, also known as Splicing factor 3B subunit 3, is part of the RNA splicing machinery. It is expressed by the Sf3b3 gene and encodes subunit 3 of the splicing factor 3b protein complex. As a component of this protein complex, it is associated with U2 snRNP and recruited to prespliceosomal complexes. Splicing factor 3b along with splicing factor 3a and a 12S RNA, form the U2 small nuclear ribonucleoprotein complex (U2 snRNP). In pre-mRNA’s, the splicing factor 3b/3a complex binds upstream of intron branch sites possibly anchoring the U2 snRNP to the pre-mRNA.
SAP130 is also a component of the STAGA (SPT3-TAF(II)31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF(II)-containing complex). These complexes are believed to function in chromatin modification, transcription, splicing, and DNA repair.

~ 136 kDa

Immunogen

Ovalbumin-conjugated linear peptide corresponding to mouse SAP130.

Application

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected SAP130 in NIH/3T3 cells.

Use Anti-SAP130 Antibody, clone 1YO 1B10 (Mouse Monoclonal Antibody) validated in WB, ICC to detect SAP130 also known as 130 kDa Sin3-associated polypeptide, SF3b130.

Biochem/physiol Actions

This antibody recognizes SAP130.

Analysis Note

Evaluated by Western Blot in RAW264.7 cell lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected SAP130 on 10 µg of RAW264.7 cell lysate.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Global Trade Item Number

SKU	GTIN
04-1549	04053252510854

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