Key Documents
04-1550
Anti-SC-35 Antibody, clone 1SC-4F11
ascites fluid, clone 1SC-4F11, from mouse
Synonym(s):
Protein PR264, Splicing component, 35 kDa, Splicing factor SC35, splicing factor, arginine/serine-rich 2
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All Photos(2)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
1SC-4F11, monoclonal
species reactivity
human, mouse, rat
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... SRSF2(6427)
General description
SC-35 is also known as Splicing factor SC35 and Splicing factor, arginine/serine-rich 2. This protein is encoded by the SFRS2 gene and is a member of the splicing factor SR family. SC-35 is involved in pre-mRNA splicing and is found in bodies in the nucleus that are highly enriched in poly(A) RNA. These nuclear bodies are referred to as speckles, SC 35 domains, or splicing factor compartments (SFCs). The purpose of these bodies is still widely disputed. Speckles correspond largely to, if not entirely to, ultra structures called inter-chromatin granule clusters (IGCs). SC3-35 is required for pre-mRNA splicing events and is highly involved in spliceosomal interaction and assembly. SC-35 is necessary for assembly of the ATP-dependent splicing complex. It is also necessary for the ATP-dependent communication between pre-mRNA and U1 and U2 snRNPs.
~ 35 kDa
Immunogen
Epitope: Unknown
Ovalbumin-conjugated linear peptide corresponding to human SC35.
Application
Anti-SC-35 Antibody, clone 1SC-4F11 is a Mouse Monoclonal Antibody for detection of SC-35 also known as Protein PR264, Splicing factor SC35 & has been validated in WB, ICC.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected SC35 in NIH/3T3 cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins
RNA Metabolism & Binding Proteins
Biochem/physiol Actions
This antibody recognizes SC35.
Physical form
Unpurified
Unpurified mouse monoclonal IgG1κ preservative-free ascites.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
A431 cell lysate
A431 cell lysate
Evaluated by Western Blot in A431 cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected Sc35 on 10 µg of A431 cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody detected Sc35 on 10 µg of A431 cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Joseph Pangallo et al.
Blood, 135(13), 1032-1043 (2020-01-22)
Genes encoding the RNA splicing factors SF3B1, SRSF2, and U2AF1 are subject to frequent missense mutations in clonal hematopoiesis and diverse neoplastic diseases. Most "spliceosomal" mutations affect specific hotspot residues, resulting in splicing changes that promote disease pathophysiology. However, a
Yongchao Liu et al.
Cells, 9(4) (2020-04-16)
The ratio control of 4R-Tau/3R-Tau by alternative splicing of Tau exon 10 is important for maintaining brain functions. In this study, we show that hnRNP A1 knockdown induces inclusion of endogenous Tau exon 10, conversely, overexpression of hnRNP A1 promotes
Ainhoa Martínez-Pizarro et al.
PLoS genetics, 14(4), e1007360-e1007360 (2018-04-24)
Phenylketonuria (PKU), one of the most common inherited diseases of amino acid metabolism, is caused by mutations in the phenylalanine hydroxylase (PAH) gene. Recently, PAH exon 11 was identified as a vulnerable exon due to a weak 3' splice site
Hanna Kędzierska et al.
International journal of molecular sciences, 17(10) (2016-10-01)
Serine and arginine rich splicing factor 2(SRSF2) belongs to the serine/arginine (SR)-rich family of proteins that regulate alternative splicing. Previous studies suggested that SRSF2 can contribute to carcinogenic processes. Clear cell renal cell carcinoma (ccRCC) is the most common subtype
Sean R Kundinger et al.
The Journal of biological chemistry, 297(5), 101306-101306 (2021-10-22)
Posttranslational modifications (PTMs) such as phosphorylation of RNA-binding proteins (RBPs) regulate several critical steps in RNA metabolism, including spliceosome assembly, alternative splicing, and mRNA export. Notably, serine-/arginine- (SR)-rich RBPs are densely phosphorylated compared with the remainder of the proteome. Previously
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 04-1550 | 04053252376511 |
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