Key Documents
04-291
Anti-erbB2 (intracellular domain) Antibody, clone 24B5
clone 24B5, Upstate®, from mouse
Synonym(s):
ERBB2
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
24B5, monoclonal
species reactivity
mouse, human
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ERBB2(2064)
mouse ... Erbb2(13866)
General description
185 kDa
Immunogen
KLH-conjugated synthetic peptide encompassing the surrounding amino acids of the intracellular domain in human erbB2
Application
Anti-erbB2 (intracellular domain) Antibody, clone 24B5 detects level of erbB2 (intracellular domain) & has been published & validated for use in ELISA & WB.
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
Biochem/physiol Actions
Recognizes the intracellular domain of erbB2 (aa 860-880).
Physical form
Format: Purified
Subsequent thiophilic adsorption and size exclusion chromatography
lyophilized in 2X PBS containing 0.09% sodium azide, PEG, and sucrose.
Preparation Note
2 years at -20°C from date of shipment. Reconstitute prior to use. Reconstitute with 1 mL H2O for 15 min at room temperature. Reconstituted aliquots should be stored frozen at -80°C. Thawed aliquots may be stored at 4°C for up to 3 months. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
EGF treated SKOV-3 lysates
EGF treated SKOV-3 lysates
Routinely evaluated by immunoblot.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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