Skip to Content
Merck

04-886

Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone A11W, rabbit monoclonal

culture supernatant, clone A11W, from rabbit

Synonym(s):

signal transducer and activator of transcription 5A, signal transducer and activator of transcription 5B, transcription factor STAT5B

Sign In to View Organizational & Contract Pricing.

Select a Size

About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Key Documents

View All Documentation
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone A11W, rabbit monoclonal, culture supernatant, clone A11W, from rabbit

biological source

rabbit

antibody form

culture supernatant

antibody product type

primary antibodies

clone

A11W, monoclonal

species reactivity

rat, chimpanzee, human, mouse, bovine

technique(s)

western blot: suitable

isotype

IgG

suitability

not suitable for immunoprecipitation

NCBI accession no.

NM_003152
NM_012448

UniProt accession no.

P42229

shipped in

dry ice

target post-translational modification

phosphorylation (pTyr694/pTyr699)

Quality Level

100

Gene Information

bovine ... Stat5A(282375)

Analysis Note

Control
PDGF treated 3T3/NIH cells
Evaluated by western blot on RIPA lysates from 3T3/NIH cells untreated and treated with PDGF.

Western Blot Analysis:
A 1:1,000-1:2,000 dilution of this antibody detected phospho-STAT5A/B (Tyr694/699) in lysates from 3T3/NIH cells treated with PDGF.

Application

Detect phospho-STAT5A/B (Tyr694/699) using this Anti-phospho-STAT5A/B (Tyr694/699) Antibody, clone A11W validated for use in WB.
Research Category
Signaling

Apoptosis & Cancer
Research Sub Category
Transcription Factors

Biochem/physiol Actions

Phospho-STAT5A/B (Tyr694/699).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The STAT proteins (signal transducer and activator of transcription) comprise a family of transcription factors. STATs are activated through tyrosine phosphorylation, mainly by JAK kinases, which results in their dimerization, nuclear translocation and regulation of gene expression. STAT5 activity is induced by numerous cytokines and growth factors. While STAT5A and STAT5B share 96% amino acid homology, the two isoforms have distinct physiological functions. Phosphorylation of tyrosine 694/699 is a key marker of STAT5A/B activation.
~95 kDa

Immunogen

Epitope: pTyr694/699
KLH-conjugated, synthetic peptide containing the sequence DGpYVK in which pY corresponds to phospho-tyrosine at residue 694 of human STAT5A or residue 699 of STAT5B.

Other Notes

Replaces: 05-886

Physical form

Cultured supernatant containing 0.05% sodium azide.

Preparation Note

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

B Sporri et al.
Blood, 97(1), 221-226 (2001-01-03)
JAB/suppressor of cytokine signaling 1 (SOCS1) STAT-induced STAT inhibitor-1 (SSI-1) (JAB/SOCS1/SSI-1) is an SH2-domain-containing protein that is induced by and negatively regulates signaling by a number of cytokines including interleukin-4 (IL-4), IL-6, interferon (IFN)-gamma, prolactin, growth hormone, and erythropoietin. The
Altered interleukin-12 responsiveness in Th1 and Th2 cells is associated with the differential activation of STAT5 and STAT1.
Gollob, J A, et al.
Blood, 91, 1341-1354 (1998)
Jennifer N Moloney et al.
Oncotarget, 8(62), 105440-105457 (2017-12-30)
Internal tandem duplication of the juxtamembrane domain of FMS-like tyrosine kinase 3 (FLT3-ITD) is the most prevalent genetic aberration present in 20-30% of acute myeloid leukaemia (AML) cases and is associated with a poor prognosis. FLT3-ITD expressing cells express elevated
Georg Pongratz et al.
Annals of the rheumatic diseases, 73(1), 306-312 (2013-03-19)
The sympathetic nervous system (SNS) as well as the interleukin (IL)-7/IL-7 receptor (IL-7R) system play a role in the pathogenesis of arthritis. However, the target cells and mechanisms involved are not fully resolved. The goal of this study was to
Pey-Jium Chang et al.
Oncotarget, 8(46), 80595-80611 (2017-11-09)
Patients with diabetes are generally prone to pathogen infection and tumor progression. Here, we investigated the potential association between diabetes and Kaposi's sarcoma (KS), a tumor linked to infection with Kaposi's sarcoma-associated herpesvirus (KSHV). By using Taiwan's National Health Insurance
View All Related Papers

Related Content

JAK/STAT Signaling Research Focus

The Janus kinase (JAK)/signal transducers and activators of transcription (STAT) signaling pathway plays an important role in cell proliferation, cell differentiation, cell migration, and cell death. It is the principal signaling mechanism for a variety of cytokines and growth factors. Constitutive activation or dysregulation of JAK/STAT signaling can result in inflammatory disease, erythrocytosis, gigantism, and leukemia.

Global Trade Item Number

SKUGTIN
04-88604053252468582

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service