05-1144
Anti-Talin Antibody, CT, clone TD77
clone TD77, from mouse
Synonym(s):
talin 1
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
TD77, monoclonal
species reactivity
chicken, human, mouse
technique(s)
immunohistochemistry: suitable (paraffin), western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
chicken ... Tln1(395194)
human ... TLN1(7094)
mouse ... Tln1(21894)
General description
270 kDa
Talin consisting of a 47-kDa N-terminal head domain (residues 1-433) and a 190-kDa
C-terminal rod domain (residues 434-2541), links integrins to the actin cytoskeleton.
C-terminal rod domain (residues 434-2541), links integrins to the actin cytoskeleton.
Application
Detect Talin using this Anti-Talin Antibody, C-terminus, clone TD77 validated for use in WB, IH(P).
Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected Talin in human GI track tissue.
Biochem/physiol Actions
Expected cross-reactivity in mouse and chicken.
Recognizes Human Talin, and cross reacts with Mouse and chicken Talin. The epitope recognized by this antibody is located within the C-terminal region of the protein (residues 2269-2541), which also contains an F-actin binding site homologous to that in the yeast actin-binding protein SIa2p.
Physical form
Format: Purified
Purified from Protein A Sepharose™ chromatography
Purified immunoglobulin from Protein A Sepharose™ chromatography. Liquid in 0.02M PBS pH 7.6, 0.25 M NaCl containing 0.1% sodium azide.
Preparation Note
Maintain between 2 and 8°C for 1 year after date of receipt.
Analysis Note
Evaluated by Western Blot in Jurkat cell lysates.
Western Blot Analysis: A 1:500 dilution of this lot detected Talin in 10 ug of Jurkat lysates.
Western Blot Analysis: A 1:500 dilution of this lot detected Talin in 10 ug of Jurkat lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MAB3264
Legal Information
Sepharose is a trademark of Cytiva
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Storage Class
10 - Combustible liquids
wgk
WGK 2
Certificates of Analysis (COA)
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