05-1352
Anti-Histone H2B Antibody, clone 5HH2-2A8
ascites fluid, clone 5HH2-2A8, from mouse
Synonym(s):
H2B, Histone H2B, histone 2, H2be, histone cluster 2, H2be, H2B histone family, member Q
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-Histone H2B Antibody, clone 5HH2-2A8, ascites fluid, clone 5HH2-2A8, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
5HH2-2A8, monoclonal
species reactivity
mouse, human, rat
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
GenBank accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... H2BC21(8349)
mouse ... H2Bc21(319190)
Application
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Anti-Histone H2B Antibody, clone 5HH2-2A8 is a Mouse Monoclonal Antibody for detection of Histone H2B also known as Histone H2B, histone 2 H2be & has been validated in WB, ICC.
Immunocytochemistry:
A 1:500 dilution of a representative lot of this antibody detected Histone H2B in A431, HeLa and NIH/3T3 cells.
A 1:500 dilution of a representative lot of this antibody detected Histone H2B in A431, HeLa and NIH/3T3 cells.
Biochem/physiol Actions
Antibody recognizes Histone H2B near the C-terminus.
Analysis Note
Control
HepG2 cell lysate, L6 cell lysate, Mouse Brain Lysate
HepG2 cell lysate, L6 cell lysate, Mouse Brain Lysate
Evaluated by western blot in HepG2 cell lysate.
Western Blot Analysis:
A 1:1,000-1:2,000 dilution of this antibody detected Histone H2B in 10 µg of HepG2 cell lysate.
Western Blot Analysis:
A 1:1,000-1:2,000 dilution of this antibody detected Histone H2B in 10 µg of HepG2 cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes. Histone H2B is one of the main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2B is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
~15 kDa
Immunogen
Epitope: C-terminus
Ovalbumin-conjugated synthetic linear peptide corresponding to the C-terminus of Histone H2B.
Other Notes
Replaces: MABE15
Physical form
Mouse monoclonal IgG1κ ascites with 0.05% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Legal Information
GenBank is a registered trademark of United States Department of Health and Human Services
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
Loading controls in western blotting application.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 05-1352 | 04053252628726 |
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