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05-1427

Sigma-Aldrich

Anti-ARF1 Antibody, clone 3F1

clone 3F1, from mouse

Synonym(s):

ADP-ribosylation factor 1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3F1, monoclonal

species reactivity

rat, human, mouse

technique(s)

ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2a

NCBI accession no.

NM_001024226.1

UniProt accession no.

P84077

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ARF1(375)
mouse ... Arf1(11840)

General description

ARF1 is a member of the ARF family of small G proteins and is a component of Golgi-derived non-clathrin coated vesicles. This antibody is class 1 ARF specific.
~21 kDa

Immunogen

Synthetic peptide corresponding to the C-terminal amino acid sequence of human ARF1.

Application

Anti-ARF1 Antibody, clone 3F1 detects level of ARF1 & has been published & validated for use in WB, ELISA, IH(P).
ELISA: A previous lot of this antibody was used in ELISA.
Optimal working dilutions must be determined by end user.

Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of ARF1 Monoclonal Antibody: Skin & Breast Cancer

Biochem/physiol Actions

Recognizes human ARF1

Physical form

Format: Purified
Purified mouse monoclonal IgG2a in 0.02 M phosphate buffer, 0.25 M NaCl, pH 7.6, with 0.1% sodium azide

Analysis Note

Routinely evaluated by Western Blot on HeLa lysates.

Western Blot Analysis: 1:500 dilution of this lot detected ARF1 on 10 μg of HeLa lysates.

Other Notes

Replaces: MAB3779

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Erica Tagliatti et al.
eLife, 5 (2016-01-06)
Recycling of synaptic vesicles (SVs) is a fundamental step in the process of neurotransmission. Endocytosed SV can travel directly into the recycling pool or recycle through endosomes but little is known about the molecular actors regulating the switch between these

Global Trade Item Number

SKUGTIN
05-142704053252277665

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