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Merck

05-1527

Anti-eIF4A3 Antibody, clone 3F1

clone 3 F 1, from mouse

Synonym(s):

ATP-dependent RNA helicase DDX48, ATP-dependent RNA helicase eIF4A-3, DEAD (Asp-Glu-Ala-Asp) box polypeptide 48, DEAD box protein 48, Eukaryotic initiation factor 4A-like NUK-34, Eukaryotic translation initiation factor 4A isoform 3, Nuclear matrix prote

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

Product Name

Anti-eIF4A3 Antibody, clone 3F1, clone 3 F 1, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

3 F 1, monoclonal

species reactivity

human

species reactivity (predicted by homology)

mouse

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NP_055555.1

UniProt accession no.

P38919

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... EIF4A3(9775)

Analysis Note

Control
HeLa cell lysate
Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected eIF4A3 on 10 µg in HeLa cell lysate.

Application

Immunoprecipitation: Reported by independent laboratory using representative lot.
Immunocytochemistry: Reported by independent laboratory using representative lot.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
RNA Metabolism & Binding Proteins
Use Anti-eIF4A3 Antibody, clone 3F1 (mouse monoclonal antibody) validated in WB, IP, ICC to detect eIF4A3 also known as ATP-dependent RNA helicase DDX48, Eukaryotic translation initiation factor 4A isoform 3.

Biochem/physiol Actions

This antibody recognizes eIF4A3.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

47 kDa
Eukaryotic translation initiation factor 4A, isoform 3, also known as eIF4A3, is a member of the DEAD box protein family. DEAD box proteins are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). This family of proteins are putative RNA helicases and are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. eIF4A3 is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family.

Immunogen

Epitope: Unknown
GST-tagged recombinant protein corresponding to human eIF4A3.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Qingke Li et al.
Translational oncology, 46, 101996-101996 (2024-05-26)
Recent studies indicate that circular RNAs (circRNAs) are crucial in the progression of colorectal cancer (CRC). Eukaryotic translation initiation factor 4A3 (EIF4A3) has been identified as a promoter of circRNA production. The biological roles and mechanisms of EIF4A3-derived circRNA (circEIF4A3)
Bo Wang et al.
Development (Cambridge, England), 148(9) (2021-04-30)
A key step in the activation of canonical Wnt signaling is the interaction between β-catenin and Tcf/Lefs that forms the transcription activation complex and facilitates the expression of target genes. Eukaryotic initiation factor 4A3 (EIF4A3) is an ATP-dependent DEAD box-family

Global Trade Item Number

SKUGTIN
05-152704053252736971

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