Key Documents

COA/COQ

05-1533

Anti-Gemin3 Antibody, clone 12H12

Name: Anti-Gemin3 Antibody, clone 12H12
Brand: MM

clone 12H12, from mouse

Synonym(s):

Component of gems 3, DEAD (Asp-Glu-Ala-Asp) box polypeptide 20, DEAD box protein 20, DEAD box protein DP 103, DEAD-box protein DP103, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 20, 103kD, SMN-interacting protein

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

biological source

mouse

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

12H12, monoclonal

species reactivity

human

technique(s)

western blot: suitable

NCBI accession no.

NP_009135.4

UniProt accession no.

Q9UHI6

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... DDX20(11218)

General description

Detects a band of approximately 105 kDa (Calculated molecular weight: 92 kDa)

Gemin3 is a member of the DEAD box protein family. The members of this family are characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). Some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Gemin 3 is a component of the survival of motor neurons (SMN) complex and codes for a DEAD box protein with an ATPase activity The SMN complex is essential for the biogenesis of spliceosomal small nuclear ribonucleoproteins and likely functions in the assembly, metabolism, and transport of a diverse number of other ribonucleoproteins. Specifically, the SMN complex assembles 7 Sm proteins (Gemin2-8) into a core structure around a highly conserved sequence of ribonucleic acid (RNA) found in small nuclear RNAs. The complex recognizes specific sequences and structural features of small nuclear RNAs and Sm proteins and assembles small nuclear ribonucleoproteins in a stepwise fashion.

Immunogen

Epitope: Unknown

Recombinant protein corresponding to human Gemin-3.

Application

Anti-Gemin3 Antibody, clone 12H12 is a Mouse Monoclonal Antibody for detection of Gemin3 also known as Component of gems 3, DEAD-box protein DP103 & has been validated in WB.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Biochem/physiol Actions

This antibody recognizes Gemin-3.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgGκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected Gemin-3 on 10 µg of HeLa cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Loss of function mutations in GEMIN5 cause a neurodevelopmental disorder.

Sukhleen Kour et al.

Nature communications, 12(1), 2558-2558 (2021-05-09)

GEMIN5, an RNA-binding protein is essential for assembly of the survival motor neuron (SMN) protein complex and facilitates the formation of small nuclear ribonucleoproteins (snRNPs), the building blocks of spliceosomes. Here, we have identified 30 affected individuals from 22 unrelated

SECIS-binding protein 2 interacts with the SMN complex and the methylosome for selenoprotein mRNP assembly and translation.

Anne-Sophie Gribling-Burrer et al.

Nucleic acids research, 45(9), 5399-5413 (2017-01-25)

Selenoprotein synthesis requires the co-translational recoding of a UGASec codon. This process involves an RNA structural element, called Selenocysteine Insertion Sequence (SECIS) and the SECIS binding protein 2 (SBP2). Several selenoprotein mRNAs undergo unusual cap hypermethylation by the trimethylguanosine synthase

Global Trade Item Number

SKU	GTIN
05-1533	04053252744082

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