Anti-Septin-6 Antibody, clone 9E7

Septin-6 is a filament-forming GTPase that participates in cytokenisis and is necessary for the normal organization of the actin cytoskeleton. It is widely expressed in tissues and is found in the cytoplasm and in the spindle during metaphase. Four isoforms of Septin-6 have been described.
As a protein family Septins were first characterized as regulators of cytokinesis. These proteins were later shown to have a broader role in microtubule-dependent processes, such as karyokinesis, exocytosis, and maintenance of cell shape. Several septin family members are known to colocalize or interact with the microtubule cytoskeleton and are believed to play a role in regulating microtubule dynamics through interactions with microtubule-associated proteins (MAPs).

~ 50 kDa

Epitope: Unknown

GST-tagged recombinant protein corresponding to human Septin-6.

Anti-Septin-6 Antibody, clone 9E7 is a Mouse Monoclonal Antibody for the detection of Septin-6 & has been validated in WB & IP.

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in IP. (Kremer, B., et al. (2005). MBC. 16(10):4648-4659.)

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Cytoskeleton

Recognizes Septin-6.

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG3κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
HeLa cell lysate

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.1 µg/ml of this antibody detected Septin-6 on 10 µg of HeLa cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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