Key Documents

COA/COQ

05-1570

Anti-PRDM1 (BLIMP-1) Antibody, clone 6D3

Name: Anti-PRDM1 (BLIMP-1) Antibody, clone 6D3
Brand: MM

clone 6D3, from rat

Synonym(s):

PR domain containing 1, with ZNF domain, PR-domain zinc finger protein 1, beta-interferon gene positive-regulatory domain I binding factor, Positive regulatory domain I-binding factor 1, PRDI-binding factor-1, Beta-interferon gene positive regulatory dom

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About This Item

UNSPSC Code:

12352203

eCl@ss:

32160702

NACRES:

NA.41

biological source

rat

Quality Level

100

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6D3, monoclonal

species reactivity

mouse, human

technique(s)

ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NP_031574

UniProt accession no.

Q60636

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PRDM1(639)

General description

B-lymphocyte induced maturation protein (BLIMP-1) is a 100 kDa transcriptional repressor containing 1 DET domain and 4 C2H2-type zinc fingers. In the nucleus, BLIMP-1 is known to bind specifically with the Beta-interferon gene positive regulatory domain I (PRDI), and to interact with PRMT5. During B-cell differentiation, BLIMP-1 actively represses the transcription of the gene, c-myc, through binding its promoter and has been deemed a “master regulator” for terminal B-cell differentiation, as it is able to initiate the BCL1 cell developmental cascade.

~ 95 kDa observed

Immunogen

Epitope: Recognizes epitope located between amino acids 255-395 of BLIMP-1

Recombinant protein corresponding to mouse BLIMP-1.

Application

ELISA Analysis: A representative lot was used by an independent laboratory in ELISA. (Kallies, A., et al. (2004). J Exp Med. 200(8):967-977.)

Flow Cytometry Analysis: A representative lot was used by an independent laboratory in FC. (Kallies, A., et al. (2004). J Exp Med. 200(8):967-977.)

Research Category
Epigenetics & Nuclear Function

Inflammation & Immunology

Use Anti-PRDM1 (BLIMP-1) Antibody, clone 6D3 (Rat Monoclonal Antibody) validated in WB, ELISA, IHC, FC to detect PRDM1 (BLIMP-1) also known as PR-domain zinc finger protein 1.

Biochem/physiol Actions

Recognizes BLIMP-1

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blot in HL-60 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected BLIMP-1 on 10 µg of HL-60 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)