05-1573
Anti-Pax5 Antibody, clone 1H9
clone 1H9, from rat
Synonym(s):
Paired box protein Pax-5, B-cell-specific transcription factor, BSAP
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
1H9, monoclonal
Species reactivity:
human, mouse
Application:
IHC, IP, WB
Citations:
3
biological source
rat
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
1H9, monoclonal
species reactivity
human, mouse
technique(s)
immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... PAX5(5079)
General description
Pax5, also known as B-cell-specific transcription factor (BASP), and encoded by the gene Pax5/Pax-5, plays a role in B cell differentiation and neural development and spermatogenesis. Pax5 regulates the expression of CD19 a B lymphoid specific target gene. Additionally, Pax5 interacts with ETS1 and alters its DNA binding abilities and it interacts with DAXX and TLE4. Pax5 is localized to the nucleus. Pax5 is expressed at early B-cell differentiation and in the developing CNS during development. In the adult it is expressed in the testis. Pax5 is also involved in B-cell related diseases and lymphomas and most recently was shown to be the controlling factor of the “Z” protein BZLF1 which controls the latent to lytic switch of Epstein-Barr virus (EBV) in humans.
~42 kDa observed
Immunogen
GST-tagged recombinant protein corresponding to mouse Pax5.
Application
Detect PAX5 using this rat monoclonal antibody, Anti-Pax5 Antibody, clone 1H9 validated for use in western blotting & IHC.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected Pax5 in 10 µg of mouse E13-E14 brain tissue lysate.
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Isocitrate dehydrogenase in human tonsil and mouse hindbrain tissue.
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Isocitrate dehydrogenase in human tonsil and mouse hindbrain tissue.
Physical form
Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Western Blotting in Ramos cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Pax5 in 10 µg of Ramos cell lysate.
Western Blotting Analysis: 0.5 µg/mL of this antibody detected Pax5 in 10 µg of Ramos cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MABE517
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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