Anti-Src Antibody, clone GD11

Proto-oncogene tyrosine-protein kinase Src (UniProt: P12931; EC:2.7.10.2, Proto-oncogene c-Src, p60-Src) is encoded by the SRC (also known as SRC1) gene (Gene ID: 6714) in human. c-Src is expressed ubiquitously and platelets, neurons, and osteoclasts express several fold higher levels than most other tissues. c-Src, is found to be over-expressed and highly activated in a wide variety of human cancers and in several tumor cell lines. Src is a non-receptor protein tyrosine kinase that is activated following engagement of many different classes of cellular receptors. Src participates in signaling pathways that control a variety of biological processes including gene transcription, immune response, cell adhesion, cell cycle progression, apoptosis, migration, and transformation. It is one of the primary kinases activated following engagement of receptors and plays a role in the activation of other protein tyrosine kinases. Its protein kinase domain is localized in amino acids 270-523. Src has a SH3 domain (aa 84-135) and a SH2 domain (aa 151-248) that are important for the intramolecular and intermolecular interactions that regulate its catalytic activity, localization, and substrate recruitment. Src can undergo phosphorylation on tyrosine 530 by c-Src kinase, which inhibits its kinase activity. Src is reported to assume an inactive conformation when this phosphotyrosine is bound by the SH2 domain in an intramolecular fashion.

A recombinant fragment expressed in E.coli corresponding to 88 amino acids from the N-terminal half of human Proto-oncogene tyrosine-protein kinase Src (c-Src).

IP-Kinase Assay: A representative lot detected Src in IP-Kinase Assay applications

Western Blotting Analysis: A representative lot detected Src in Western Blotting applications

mmunoprecipitation Analysis: A representative lot detected Src in Immunoprecipitation applications

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Clone GD11 is a mouse monoclonal antibody that detects Proto-oncogene tyrosine-protein kinase Src (c-Src). It targets an epitope within 88 amino acids from the N-terminal half.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Stable for 1 year at +2°C to +8°C from date of receipt.

Evaluated by Western Blotting in A431 cell lysates.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

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