05-253

Anti-ZAP-70 Antibody, clone 2F3.2

Name: Anti-ZAP-70 Antibody, clone 2F3.2
Brand: MM

clone 2F3.2, Upstate^®, from mouse

Synonym(s):

Anti-ADMIO2, Anti-SRK, Anti-STCD, Anti-STD, Anti-TZK, Anti-ZAP-70

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Product Name

Anti-ZAP-70 Antibody, clone 2F3.2, clone 2F3.2, Upstate^®, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

2F3.2, monoclonal

species reactivity

human

manufacturer/tradename

Upstate^®

technique(s)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2a

NCBI accession no.

NM_001079

UniProt accession no.

P43403

shipped in

dry ice

target post-translational modification

unmodified

Physical form

Protein G Purified immunoglobulin in Protein G Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

Format: Purified

Protein G Purified

Preparation Note

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Analysis Note

Control
Positive Antigen Control: Catalog #12-303, Jurkat cell lysate.

routinely evaluated on RIPA lysates prepared from Jurkat cells or Hut-78 cells

Application

For testing in IHC, please see the following link (PDF): http://www.propathlab.com/pdf/focus_dec_2003.pdf

Research Category
Signaling

Research Sub Category
Immunological Signaling

This Anti-ZAP-70 Antibody, clone 2F3.2 is validated for use in FC, IH, IP, WB for the detection of ZAP-70.

Biochem/physiol Actions

Recognizes human ZAP-70, does not recognize murine ZAP-70.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

70 kDa

Note: This product is for research use only. It is not manufacured for diagnostic or therapeutic use in humans or animals.

Zeta-chain-associated protein kinase 70. A member of the protein tyrosine kinase family, ZAP-70 is normally expressed in T cells and natural killer cells and has a critical role in the initiation of T-cell signaling. ZAP-70 is expressed in T cells and tumors of T-cell lineage. A high level of ZAP-70 expression appears restricted to T-cell proliferative diseases and a subgroup of chronic lymphocytic leukemia (CLL). The ZAP-70 gene is in chromosome 2q12.

Immunogen

GST-fusion to tandem SH2 domains of human ZAP-70 corresponding to residues 1-254

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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ZAP-70 promotes the infiltration of malignant B-lymphocytes into the bone marrow by enhancing signaling and migration after CXCR4 stimulation.

Eva Calpe et al.

PloS one, 8(12), e81221-e81221 (2013-12-07)

ZAP-70 in chronic lymphocytic leukemia (CLL) is associated with enhanced response to microenvironmental stimuli. We analyzed the functional consequences of ZAP-70 ectopic expression in malignant B-cells in a xenograft mouse model of disseminated B-cell leukemia. Mice injected with B-cells expressing

A role for Wiskott-Aldrich syndrome protein in T-cell receptor-mediated transcriptional activation independent of actin polymerization.

C Silvin et al.

The Journal of biological chemistry, 276(24), 21450-21457 (2001-04-03)

Wiskott-Aldrich syndrome protein (WASP) plays a key role in cytoskeletal rearrangement and transcriptional activation in T-cells. Recent evidence links WASP and related proteins to actin polymerization by the Arp2/3 complex. To study whether the role of WASP in actin polymerization

Genetic evidence of a role for Lck in T-cell receptor function independent or downstream of ZAP-70/Syk protein tyrosine kinases

Wong, J., et al

Molecular and cellular biology, 18, 2855-2866 (1998)

Defective expression of the T-cell receptor-CD3 zeta chain in T-cell acute lymphoblastic leukaemia.

Giovanni F Torelli, Rossella Paolini, Caterina Tatarelli, Alessandra Soriani et al.

British Journal of Haematology null

Requirement for Tec kinases Rlk and Itk in T cell receptor signaling and immunity.

E M Schaeffer et al.

Science (New York, N.Y.), 284(5414), 638-641 (1999-04-24)

T cell receptor (TCR) signaling requires activation of Zap-70 and Src family tyrosine kinases, but requirements for other tyrosine kinases are less clear. Combined deletion in mice of two Tec kinases, Rlk and Itk, caused marked defects in TCR responses

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Global Trade Item Number

SKU	GTIN
05-253	04053252330896

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