Key Documents
05-312
Anti-E2F-4 Antibody, clone GG22-2A6
clone GG22-2A6, Upstate®, from mouse
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
GG22-2A6, monoclonal
species reactivity
rat, mouse, human
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2b
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... E2F4(1874)
General description
55kDa
Immunogen
GST fusion to human E2F-4
Application
Detect E2F-4 with Anti-E2F-4 Antibody, clone GG22-2A6 (Mouse Monoclonal Antibody), that has been shown to work in IP & WB.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors
Transcription Factors
Biochem/physiol Actions
E2F-4; does not recognize E2F-1, -2, -3, or -5
Physical form
200μg of protein G purified mouse IgG2b in 200μl of 0.07 M Tris-glycine, pH 7.4, 0.135 M NaCl, 0.035% sodium azide and 30% glycerol.
Format: Purified
Protein G Chromatography
Preparation Note
1 year at -20°C
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
routinely evaluated on non-stimulated A431 cell lysate
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Functional interaction between E2F-4 and p130: evidence for distinct mechanisms underlying growth suppression by different retinoblastoma protein family members.
Vairo, G, et al.
Genes & Development, 9, 869-881 (1995)
L Wu et al.
Molecular and cellular biology, 20(19), 7059-7067 (2000-09-13)
Expression of the bovine papillomavirus E2 protein in cervical carcinoma cells represses expression of integrated human papillomavirus (HPV) E6/E7 oncogenes, followed by repression of the cdc25A gene and other cellular genes required for cell cycle progression, resulting in dramatic growth
Dynamic loss of H2B ubiquitylation without corresponding changes in H3K4 trimethylation during myogenic differentiation.
Vethantham, V; Yang, Y; Bowman, C; Asp, P; Lee, JH; Skalnik, DG; Dynlacht, BD
Molecular and cellular biology null
Chang-Rung Chen et al.
Cell, 110(1), 19-32 (2002-08-02)
Smad3 is a direct mediator of transcriptional activation by the TGFbeta receptor. Its target genes in epithelial cells include cyclin-dependent kinase inhibitors that generate a cytostatic reponse. We defined how, in the same context, Smad3 can also mediate transcriptional repression
Erawan Borkham-Kamphorst et al.
Journal of cell communication and signaling, 6(1), 11-25 (2011-07-13)
Nephroblastoma overexpressed gene encodes a matricellular protein (CCN3/NOV) of the CCN family, comprising CCN1 (CYR61), CCN2 (CTGF), CCN4 (WISP-1), CCN5 (WISP-2), and CCN6 (WISP-3). CCN proteins are involved in the regulation of mitosis, adhesion, apoptosis, extracellular matrix production, growth arrest
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 05-312 | 04053252280740 |
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