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Key Documents

05-351

Sigma-Aldrich

Anti-Fas Antibody

UPSTATE®, rat monoclonal, 7C10

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Product Name

Anti-Fas Antibody, clone 7C10, clone 7C10, Upstate®, from rat

biological source

rat

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

7C10, monoclonal

species reactivity

mouse, bovine

manufacturer/tradename

Upstate®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2a

NCBI accession no.

NM_152877

UniProt accession no.

P25445

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

bovine ... Fas(282488)

General description

45kDa

Specificity

Fas

Immunogen

recombinant protein containing the cytoplasmic domain of mouse Fas

Application

Anti-Fas Antibody, clone 7C10 is an antibody against Fas for use in IP & WB.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Physical form

0.1 M Tris-glycine, 0.15 M NaCl, pH 7.4 with 0.05% sodium azide
Format: Purified
Protein G Chromatography

Preparation Note

2 years at -20°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-305, 3T3/A31 lysate. Add 2.5 μL of 2-mercapto-ethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.
routinely evaluated on RIPA lysates of mouse 3T3/A31 fibroblasts or membrane preparations of bovine brain

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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