05-369X-555
Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6, Alexa Fluor™ 555
clone C464.6, Upstate®, from mouse
Synonym(s):
Anti-CMT2DD
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
ALEXA FLUOR™ 555
Clone:
C464.6, monoclonal
Application:
FACS, IF, WB
Citations:
1
biological source
mouse
Quality Level
conjugate
ALEXA FLUOR™ 555
antibody form
purified antibody
antibody product type
primary antibodies
clone
C464.6, monoclonal
species reactivity
mouse, human, rabbit, Xenopus, sheep, monkey, canine, pig, rat
packaging
antibody small pack of 25 μg
manufacturer/tradename
Upstate®
technique(s)
flow cytometry: suitable, immunofluorescence: suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ATP1A3(478)
General description
113 kDa
Immunogen
Purified Na+/K+ ATPase isolated from membrane fractions of rabbit kidney outer medulla.
Application
Detect Na+/K+ ATPase α-1 using this Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6, Alexa Fluor 555 validated for use in FC, IF & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion Channels & Transporters
Ion Channels & Transporters
Biochem/physiol Actions
Specific for the α-1 subunit isoform.
Physical form
25µg Alexa Fluor™ 555-conjugated protein G purified mouse IgG1κ in 100µl of PBS containing 1% BSA, 0.05% Tween-20, 0.05% sodium azide. Liquid at 4°C.
Protein G Purified
Preparation Note
Do Not Freeze. Do not store the material diluted. Stable for 1 year at 4°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing cap.
Analysis Note
Control
Included Negative Control: Catalog # 16-239, Alexa Fluor™ 555-conjugated Normal Mouse IgG.
Included Negative Control: Catalog # 16-239, Alexa Fluor™ 555-conjugated Normal Mouse IgG.
Immunoblot Analysis: 2µg/ml of this lot detected α-1 subunit in 40μg of rat brain microsomal preparation.
Immunocytochemistry: 2µg/ml of this lot showed positive immunostaining for α-1 subunit in Colo 205 cells.
Flow Cytometry: 0.2μg of this lot detected α-1 subunit in fixed NCI H292 cells (see data).
Immunocytochemistry: 2µg/ml of this lot showed positive immunostaining for α-1 subunit in Colo 205 cells.
Flow Cytometry: 0.2μg of this lot detected α-1 subunit in fixed NCI H292 cells (see data).
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Alexa Fluor™ is a registered trademark of Molecular Probes, Inc.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Carles Barceló et al.
Journal of cell science, 126(Pt 20), 4553-4559 (2013-08-15)
Activating mutations in the K-Ras small GTPase are extensively found in human tumors. Although these mutations induce the generation of a constitutively GTP-loaded, active form of K-Ras, phosphorylation at Ser181 within the C-terminal hypervariable region can modulate oncogenic K-Ras function
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 05-369X-555 | 04053252277658 |