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Key Documents

05-516

Sigma-Aldrich

Anti-KRAS Antibody

UPSTATE®, mouse monoclonal, RAS10

Synonym(s):

GTP- and GDP-binding peptide B, GTPase Hras, Ha-Ras1 proto-oncoprotein, Ras family small GTP binding protein H-Ras, Transforming protein p2, c-has/bas p21 protein, c-ras-Ki-2 activated oncogene, p19 H-RasIDX protein, transformation gene: oncogene HAMSV,

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All Photos(4)

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

Product Name

Anti-Ras Antibody, clone RAS10, clone RAS10, Upstate®, from mouse

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

RAS10, monoclonal

species reactivity

rat, human, mouse

manufacturer/tradename

Upstate®

technique(s)

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

NM_005343

UniProt accession no.

P01116

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... HRAS(3265), KRAS(3845), NRAS(4893)

Related Categories

General description

The tumor oncoproteins HRas, KRas, and NRas are rated as Ras protoncogenes. Point mutations within Ras genes are frequently detected in human malignancies and in experimentally induced tumors in animals.
21 kDa

Immunogen

Purified recombinant Ras. Clone RAS10.

Application

Detect Ras using this Anti-Ras Antibody, clone RAS10 validated for use in ELISA, FC, IC, IH, IP & WB.
Immunoprecipitation:
1-4 μg of this antibody has been reported to immunoprecipitate Ras.

Immunocytochemistry:
2.5 μg/mL of this antibody has been reported to show positive immunostaining for Ras in SW480 and Y1 cells.

Immunohistochemistry:
This antibody has been reported to detect Ras in frozen and paraffin embedded breast carcinoma sections.

Biochem/physiol Actions

Other species unknown.

Physical form

Format: Purified
Purified mouse monoclonal IgG2ak in buffer containing PBS, pH 7.4, with 0.05% sodium azide and 30% glycerol. Liquid at -20°C.

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Routinely evaluated by western blot on RIPA lysate from human A431 carcinoma cells, human HFF, Jurkat, Raji, mouse 3T3, CTLL, rat L-6 or PC-12 cell lysates.

Western Blot Analysis:
0.05 - 0.5 μg/mL of this lot detected Ras in RIPA lysate from human A431 carcinoma cells. A previous lot detected Ras in human HFF, Jurkat, Raji, mouse 3T3, CTLL, rat L-6 and PC- 12 cell lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: CBL751

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Oncogenic Ras and its role in tumor cell invasion and metastasis.
Campbell, Paul M and Der, Channing J
Seminars in cancer biology, 14, 105-114 (2004)
GTP hydrolysis mechanism of Ras-like GTPases.
Li, Guangpu and Zhang, Xuejun C
Journal of Molecular Biology, 340, 921-932 (2004)
Ras plasma membrane signalling platforms.
Hancock, John F and Parton, Robert G
The Biochemical Journal, 389, 1-11 (2005)
Oncogenic Ras in tumour progression and metastasis.
Giehl, Klaudia
Biological Chemistry, 386, 193-205 (2005)
Requirement of Cdk4 for v-Ha-ras-Induced Breast Tumorigenesis and Activation of the v-ras-Induced Senescence Program by the R24C Mutation.
Reddy, HK; Gra?a, X; Dhanasekaran, DN; Litvin, J; Reddy, EP
Genes & Cancer null
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Global Trade Item Number

SKUGTIN
05-51604053252732614

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