Key Documents
05-572
Anti-Caspase 9 Antibody, clone 96-2-22
clone 96-2-22, Upstate®, from mouse
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
96-2-22, monoclonal
monoclonal
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... CASP9(842)
General description
46/34 kDa
Immunogen
The N-terminal fragment of human Caspase 9 (amino acid residues 1-134)
Application
This Anti-Caspase 9 Antibody, clone 96-2-22 is validated for use in WB for the detection of Caspase 9.
Biochem/physiol Actions
Recognizes the proform and the active cleaved form of Caspase 9.
Physical form
Format: Purified
Protein A Purified immunoglobulin in Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
routinely evaluated by immunoblot on RIPA lysates from A431, Raji, or HFF cells
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-443
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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J Rodriguez et al.
Genes & development, 13(24), 3179-3184 (2000-01-05)
Autocatalytic activation of initiator caspases is the link between pro-apoptotic signals and the destruction machinery of apoptosis. Activation of caspase-9, which mediates oncogene and drug-induced apoptosis, requires binding to the protein APAF-1. We found that the proteolytic activity of caspase-9
Cell cycle arrest and proapoptotic effects of the anticancer cyclodepsipeptide serratamolide (AT514) are independent of p53 status in breast cancer cells.
Vanessa Soto-Cerrato, Beatriz Montaner, Marc Martinell, Marta Vilaseca, Ernest Giralt et al.
Biochemical Pharmacology null
Giuseppe Filomeni et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 17(1), 64-66 (2002-11-09)
Changes in the intracellular reduced/oxidized glutathione ratio (GSH/GSSG) are crucial reduction-oxidation (redox) events that trigger downstream proliferation or death responses. We investigated the molecular mechanisms underlying redox-mediated cell signaling upon an oxidative insult by treating U937 cells with exogenous nonpermeable
BITC Sensitizes Pancreatic Adenocarcinomas to TRAIL-induced Apoptosis.
Wicker CA, Sahu RP, Kulkarni-Datar K, Srivastava SK, Brown TL
Cancer growth and metastasis null
Heat shock protein inhibitors increase the efficacy of measles virotherapy.
Liu, C; Erlichman, C; McDonald, CJ; Ingle, JN; Zollman, P; Iankov, I; Russell, SJ; Galanis, E
Gene Therapy null
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