05-716
Anti-Myosin Heavy Chain Antibody, clone A4.1025
ascites fluid, clone A4.1025, Upstate®
Synonym(s):
Myosin heavy chain 1, Myosin heavy chain 2x, Myosin heavy chain IIx/d, Myosin heavy chain, skeletal muscle, adult 1, myosin, heavy chain 1, skeletal muscle, adult, myosin, heavy polypeptide 1, skeletal muscle, adult
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
antibody form
ascites fluid
antibody product type
primary antibodies
clone
A4.1025, monoclonal
species reactivity
mouse, zebrafish, rat, Drosophila, rabbit, human
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... MYH1(4619)
General description
Myosin is one of the two major components of muscle tissue responsible for contraction. In muscle cells myosin is arranged as thick filamentous structures that are arranged in parallel to thin filamentous structures composed of actin. Muscle contraction is actuated by the coupling and uncoupling of thin and thick filaments through a chemical linkage mediated by ATP. The coupling and uncoupling is arranged such that the filaments slide in one direction when coupled and in reverse direction when uncoupled or released. Myosin and actin also serve to facilitate motility in non-muscle cells such as in slime molds where shorter yet similar filamentous structures work together. An increasing number of different myosins are being described. Myosin I is a low molecular weight myosin found in protozoa and does not self-assemble. Myosin II is the classic form found in muscle cells that self assemble into thick bundles. Myosin II is a multimeric protein that is composed of two heavy chains and two light chains.
~220 kDa
Immunogen
Partially purified protein from adult human skeletal muscle myosin. Clone A4.1025.
Application
Anti-Myosin Heavy Chain Antibody, clone A4.1025 is an antibody against Myosin Heavy Chain for use in IH & WB.
Immunohistochemistry:
This antibody has been reported by an independent laboratory to detect myosin heavy chain in zebrafish embryos and skeletal muscle sections fixed with 4% paraformaldehyde in a previous lot (Blagden, C.S., 1997; Cho, M., 1994).
This antibody has been reported by an independent laboratory to detect myosin heavy chain in zebrafish embryos and skeletal muscle sections fixed with 4% paraformaldehyde in a previous lot (Blagden, C.S., 1997; Cho, M., 1994).
Biochem/physiol Actions
This antibody recognizes myosin heavy chain, Mr ~220 kDa. Immunoblotting with muscle cell lysates will often show several close bands from different myosin heavy chain genes, as well as degradation products.
Physical form
Unpurified mouse ascites monoclonal IgG in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol. Liquid at -20°C.
Preparation Note
Stable for 1 year at -20ºC from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.
Analysis Note
Control
RIPA lysates from C2C12 cells
RIPA lysates from C2C12 cells
Routinely evaluated by Western Blot on RIPA lysates from C2C12 cells.
Western Blot Analysis:
1:1000-1:5000 dilution of this lot detected Myosin Heavy Chain (MyHC) in RIPA lysates from Rabbit skeletal muscle cells.
Western Blot Analysis:
1:1000-1:5000 dilution of this lot detected Myosin Heavy Chain (MyHC) in RIPA lysates from Rabbit skeletal muscle cells.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
Mechanical stress triggers cardiomyocyte autophagy through angiotensin II type 1 receptor-mediated p38MAP kinase independently of angiotensin II.
Lin, L; Tang, C; Xu, J; Ye, Y; Weng, L; Wei, W; Ge, J; Liu, X; Zou, Y
Testing null
A M Maggs et al.
Journal of muscle research and cell motility, 21(2), 101-113 (2000-08-29)
The contractile properties of muscle fibres are, in part, determined by the myosin heavy chain (MyHC) isoforms they express. Using monoclonal antibodies, we show that at least three forms of slow twitch MyHC accumulate sequentially during mouse fetal development and
Notochord induction of zebrafish slow muscle mediated by Sonic hedgehog
Blagden, C. S., et al
Genes & Development, 11, 2163-2175 (1997)
High-density lipoprotein inhibits mechanical stress-induced cardiomyocyte autophagy and cardiac hypertrophy through angiotensin II type 1 receptor-mediated PI3K/Akt pathway.
Lin, L; Liu, X; Xu, J; Weng, L; Ren, J; Ge, J; Zou, Y
Journal of Cellular and Molecular Medicine null
Fast myosin heavy chains expressed in secondary mammalian muscle fibers at the time of their inception
Cho, M, et al
Journal of Cell Science, 107 ( Pt 9), 2361-2371 (1994)
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