05-781R
Anti-p70 S6 Kinase Antibody, clone 20-10C-6, rabbit monoclonal
clone 20-10C-6, from rabbit, unconjugated
Synonym(s):
70 kDa ribosomal protein S6 kinase 1, Ribosomal protein S6 kinase I, p70 S6 kinase alpha, p70 S6 kinase, alpha 1, p70 S6 kinase, alpha 2, ribosomal protein S6 kinase, 70kD, polypeptide 1, ribosomal protein S6 kinase, 70kDa, polypeptide 1, serine/threonin
Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-p70 S6 Kinase Antibody, clone 20-10C-6, rabbit monoclonal, clone 20-10C-6, from rabbit, unconjugated
biological source
rabbit
conjugate
unconjugated
antibody product type
primary antibodies
clone
20-10C-6, monoclonal
species reactivity
human, mouse, rat
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Quality Level
Gene Information
human ... RPS6KB1(6198)
Analysis Note
Control
NIH/3T3, Hek293, and PC12 cell lysates
NIH/3T3, Hek293, and PC12 cell lysates
Evaluated by Western Blot in NIH/3T3, Hek293, and PC12 cell lysates.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected p70 S6 Kinase on NIH/3T3, Hek293 and PC12 cell lysates.
Western Blot Analysis: A 1:1,000 dilution of this antibody detected p70 S6 Kinase on NIH/3T3, Hek293 and PC12 cell lysates.
Application
Research Category
Signaling
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
PI3K, Akt, & mTOR Signaling
This Anti-p70 S6 Kinase Antibody, clone 20-10C-6 is validated for use in WB, IP, IC for the detection of p70 S6 Kinase.
Western Blot (SNAP ID) Analysis: A 1:250 dilution from a previous lot detected p70 S6 Kinase on NIH/3T3, Hek293, and PC12 cell lysates.
Immunoprecipiitation Analysis: 1 µg from a previous lot immunoprecipitated p70 S6 Kinase from 100 µg of HEK293 RIPA lysate.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected p70 S6 Kinase in HeLa cells.
Immunoprecipiitation Analysis: 1 µg from a previous lot immunoprecipitated p70 S6 Kinase from 100 µg of HEK293 RIPA lysate.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected p70 S6 Kinase in HeLa cells.
Biochem/physiol Actions
This antibody recognizes p70 S6 Kinase at the N-terminus.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
60 kDa
S6 Kinase is a Ser/Thr kinase which phosphorylates the 40S ribosomal protein S6, and several translation-regulatory factors. S6 kinase is activated by phosphorylation of multiple sites by mTOR. S6K is an important regulator of protein synthesis, along with MAP Kinase. In neurons, S6K is linked to the cytoskeleton by neurabin, which may play a role in its activation. Rsk3 (MAPKAP Kinase 1c) and Rsk4 are also known as the Ribosomal Protein S6 Kinase, 90 kDa, polypeptide 2 and 6, respectively.
Immunogen
Epitope: N-terminus
KLH-conjugated linear peptide corresponding to human p70 S6 Kinase at and around the N-terminus.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: 04-391
Physical form
Protein A purified
Purified rabbit IgG supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide with 40% Glycerol.
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Not finding the right product?
Try our Product Selector Tool.
Storage Class
10-13 - German Storage Class 10 to 13
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Hongwei Zhang et al.
International journal of molecular medicine, 32(4), 851-859 (2013-07-24)
Intestinal ischemia/reperfusion (I/R) injury is a serious condition in intensive care patients, resulting in severe inflammation and remote organ damage. The activation of the mammalian target of rapamycin (mTOR)/p70 ribosomal S6 kinase (p70S6K) signaling pathway exerts protective effect against ischemia/reperfusion
Fuan Wang et al.
Nature immunology, 17(5), 514-522 (2016-04-05)
Cytosolic DNA-mediated activation of the transcription factor IRF3 is a key event in host antiviral responses. Here we found that infection with DNA viruses induced interaction of the metabolic checkpoint kinase mTOR downstream effector and kinase S6K1 and the signaling
Mathilde Calvez et al.
Journal of neurovirology, 25(3), 384-396 (2019-02-14)
Despite the fact that human immunodeficiency virus type 1 (HIV-1) does not enter or replicate in neurons, its infection of a subset of resident brain glia cells (microglia and astrocytes) induces via disparate mechanisms, dysregulation of glutamate metabolism, neurotoxicity, and
Silvia Ramírez-Peinado et al.
The Journal of biological chemistry, 288(42), 30387-30398 (2013-09-10)
In response to nutrient shortage or organelle damage, cells undergo macroautophagy. Starvation of glucose, an essential nutrient, is thought to promote autophagy in mammalian cells. We thus aimed to determine the role of autophagy in cell death induced by glucose
Ferran Medina-Jover et al.
FEBS letters, 594(19), 3200-3215 (2020-08-09)
BMP9 is a cytokine involved in the maturation phase of the angiogenic process that signals through its serine/threonine receptor ALK1 and its coreceptor endoglin. In this paper, we explain how BMP9 directs the regulation of endothelial cell proliferation blockage while
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service