Anti-p70 S6 Kinase Antibody, clone 20-10C-6, rabbit monoclonal

60 kDa

S6 Kinase is a Ser/Thr kinase which phosphorylates the 40S ribosomal protein S6, and several translation-regulatory factors. S6 kinase is activated by phosphorylation of multiple sites by mTOR. S6K is an important regulator of protein synthesis, along with MAP Kinase. In neurons, S6K is linked to the cytoskeleton by neurabin, which may play a role in its activation. Rsk3 (MAPKAP Kinase 1c) and Rsk4 are also known as the Ribosomal Protein S6 Kinase, 90 kDa, polypeptide 2 and 6, respectively.

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to human p70 S6 Kinase at and around the N-terminus.

Research Category
Signaling

Research Sub Category
PI3K, Akt, & mTOR Signaling

This Anti-p70 S6 Kinase Antibody, clone 20-10C-6 is validated for use in WB, IP, IC for the detection of p70 S6 Kinase.

Western Blot (SNAP ID) Analysis: A 1:250 dilution from a previous lot detected p70 S6 Kinase on NIH/3T3, Hek293, and PC12 cell lysates.

Immunoprecipiitation Analysis: 1 µg from a previous lot immunoprecipitated p70 S6 Kinase from 100 µg of HEK293 RIPA lysate.

Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected p70 S6 Kinase in HeLa cells.

This antibody recognizes p70 S6 Kinase at the N-terminus.

Protein A purified

Purified rabbit IgG supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide with 40% Glycerol.

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Control
NIH/3T3, Hek293, and PC12 cell lysates

Evaluated by Western Blot in NIH/3T3, Hek293, and PC12 cell lysates.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected p70 S6 Kinase on NIH/3T3, Hek293 and PC12 cell lysates.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-391

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.