05-785
Anti-CD3ε Antibody, clone APA1/1
clone APA1/1, Upstate®, from mouse
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
APA1/1, monoclonal
Application:
FACS, ICC, IP, WB
Citations:
6
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
APA1/1, monoclonal
species reactivity
mouse, human
manufacturer/tradename
Upstate®
technique(s)
flow cytometry: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CD3E(916)
General description
~23kDa
Immunogen
Purified human CD3 epsilon
Application
Clone APA1/1 has been demonstrated to recognize a site on CD3&epsilon: that is exposed upon engagement of the T-Cell Receptor complex. Exposure of this neoepitope precedes CD3 phosphorylation and recruitment and activation of ZAP-70, which initiates the signaling cascade produced by T-cell activation. As such, clone APA1/1 provides the earliest known marker for T-Cell Receptor engagement, and thus T-cell activation.
Detect CD3ε using this Anti-CD3ε Antibody, clone APA1/1 validated for use in FC, IP, WB & IC.
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Immunoglobulins & Immunology
Biochem/physiol Actions
CDε
Physical form
0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide
Format: Purified
Protein G Purified
Preparation Note
1 year at 2-8°C from date of shipment.
Analysis Note
routinely evaluated by immunoblot on RIPA lysates from Jurkat cells
Other Notes
Replaces: 04-460
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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