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Key Documents

05-785

Sigma-Aldrich

Anti-CD3ε Antibody, clone APA1/1

clone APA1/1, Upstate®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

APA1/1, monoclonal
monoclonal

species reactivity

mouse, human

manufacturer/tradename

Upstate®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

NM_000733

UniProt accession no.

P07766

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CD3E(916)

General description

~23kDa

Immunogen

Purified human CD3 epsilon

Application

Clone APA1/1 has been demonstrated to recognize a site on CD3&epsilon: that is exposed upon engagement of the T-Cell Receptor complex. Exposure of this neoepitope precedes CD3 phosphorylation and recruitment and activation of ZAP-70, which initiates the signaling cascade produced by T-cell activation. As such, clone APA1/1 provides the earliest known marker for T-Cell Receptor engagement, and thus T-cell activation.
Detect CD3ε using this Anti-CD3ε Antibody, clone APA1/1 validated for use in FC, IP, WB & IC.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Biochem/physiol Actions

CDε

Physical form

0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide
Format: Purified
Protein G Purified

Preparation Note

1 year at 2-8°C from date of shipment.

Analysis Note

routinely evaluated by immunoblot on RIPA lysates from Jurkat cells

Other Notes

Replaces: 04-460

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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