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Key Documents

05-801

Sigma-Aldrich

Anti-trimethyl-Histone H3 (Lys36) Antibody, clone MC86, rabbit monoclonal

clone MC86, Upstate®, from rabbit

Synonym(s):

H3K36me3, Histone H3 (tri methyl K36)

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MC86, monoclonal

species reactivity

chicken

manufacturer/tradename

Upstate®

technique(s)

ChIP: suitable
dot blot: suitable
inhibition assay: suitable (peptide)
western blot: suitable

isotype

IgG

NCBI accession no.

NP_003484

UniProt accession no.

Q16695

shipped in

wet ice

target post-translational modification

trimethylation (Lys36)

General description

~17 kDa

Immunogen

peptide containing the sequence GVme3KKP, in which me3K corresponds to trimethyl lysine 36 of human histone H3

Application

Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones
Use Anti-trimethyl-Histone H3 (Lys36) Antibody, clone MC86 (rabbit monoclonal antibody) validated in DB, WB, PIA to detect trimethyl-Histone H3 (Lys36) also known as H3K36me3, Histone H3 (tri methyl K36).

Biochem/physiol Actions

Broad species cross-reactivity is expected.
Recognizes Histone H3 trimethylated on Lysine 36.

Physical form

Format: Purified
Protein A Purified immunoglobulin in a solution containing 0.07M Tris-glycine, 0.105M NaCl, pH 7.4, 0.035% sodium azide, 30% glycerol.
Protein A purified

Preparation Note

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Acid extracted proteins from HeLa cells
routinely evaluated by immunoblot on chicken Core Histones (Catalog #13-107); did not detect unmodified recombinant Histone H3 (Catalog #14-411)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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