Key Documents
05-858
Anti-Histone H4 Antibody, pan, clone 62-141-13, rabbit monoclonal
clone 62-141-13, Upstate®, from rabbit
Synonym(s):
H4, Histone H4, H4 histone family, member A, histone 1, H4a, histone cluster 1, H4a
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
62-141-13, monoclonal
species reactivity
chicken, Saccharomyces cerevisiae, vertebrates, yeast, human
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable (ChIP-seq)
multiplexing: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
Saccharomyces cerevisiae ... Hhf1(852294)
human ... H4C1(8359)
General description
10 kDa
Histone H4 is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H4 is involved with the structure of the nucleosomes of the ′beads on a string′ structure.
This antibody recognizes histone H4 independant of any post-translational modifications.
Immunogen
Epitope: a.a. 25-28
KLH-conjugated, synthetic peptide corresponding to amino acids 17-28 of Histone H4. Epitope has been mapped to residues 25-28.
Application
Anti-Histone H4 Antibody, pan, clone 62-141-13 is a Rabbit Monoclonal Antibody for detection of Histone H4 also known as Histone H4 & has been validated in ChIP, WB, Mplex, ChIP-seq.
Epitope Mapping (using Luminex®):
1:250-1:4,000 serial dilutions of a previous lot were incubated with histone peptides containing various unmodified peptides spanning the amino-terminus of Histone H4 conjugated to Luminex microspheres. The antibody recognized both modified and unmodified peptides (See Figure B Page 2).
Chromatin Immunoprecipitation:
An independent laboratory used a previous lot of this antibody in chromatin immunoprecipitation (Issac, C., 2006).
1:250-1:4,000 serial dilutions of a previous lot were incubated with histone peptides containing various unmodified peptides spanning the amino-terminus of Histone H4 conjugated to Luminex microspheres. The antibody recognized both modified and unmodified peptides (See Figure B Page 2).
Chromatin Immunoprecipitation:
An independent laboratory used a previous lot of this antibody in chromatin immunoprecipitation (Issac, C., 2006).
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
Biochem/physiol Actions
Broad species cross-reactivity expected based on sequence homology.
Recognizes Histone H4, Mr 10 kDa.
Physical form
Format: Purified
Protein A purified
Purified rabbit monoclonal IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated).
Acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated).
Routinely evaluated by western blot.
Western Blot Analysis:
A 1:30,000-1:100,000 dilution of this lot detected unmodified recombinant Histone H4 protein (Catalog # 14-412) and Histone H4 in acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated)
Western Blot Analysis:
A 1:30,000-1:100,000 dilution of this lot detected unmodified recombinant Histone H4 protein (Catalog # 14-412) and Histone H4 in acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated)
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
Luminex is a registered trademark of Luminex Corp
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 05-858 | 04053252404337 |
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