05-939
Anti-LSD1/BHC110 Antibody
ascites fluid, Upstate®
Synonym(s):
BRAF35-HDAC complex protein BHC110, FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit, Flavin-containing amine oxidase domain-containing protein 2, amine oxidase (flavin containing) domain 2, lysine (K)-specific demethylase 1, lysine-specific hist
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-LSD1/BHC110 Antibody, ascites fluid, Upstate®
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
monoclonal
species reactivity
mouse, human
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... KDM1A(23028)
Application
Anti-LSD1/BHC110 Antibody is a Mouse Monoclonal Antibody for detection of LSD1/BHC110 also known as BRAF35-HDAC complex protein BHC110, lysine (K)-specific demethylase 1 & has been validated in ChIP, IF, IHC, IP & WB.
Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate LSD1 from chromatin.
Immunoprecipitation:
Recommended.
Immunohistochemistry:
This antibody has been reported by an independent laboratory to detect LSD1 using paraffin-embedded tissues. (Metzger, E., 2005)
Immunofluorescence:
Recommended.
This antibody has been reported by an independent laboratory to immunoprecipitate LSD1 from chromatin.
Immunoprecipitation:
Recommended.
Immunohistochemistry:
This antibody has been reported by an independent laboratory to detect LSD1 using paraffin-embedded tissues. (Metzger, E., 2005)
Immunofluorescence:
Recommended.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Chromatin Biology
Histones
Chromatin Biology
Analysis Note
Control
HeLa cell lysate.
HeLa cell lysate.
Routinely evaluated by western blot.
Western Blot Analysis:
1:500-1:2000 dilution of this lot detected LSD1 in RIPA lysates from HeLa cells.
Western Blot Analysis:
1:500-1:2000 dilution of this lot detected LSD1 in RIPA lysates from HeLa cells.
Biochem/physiol Actions
Recognizes LSD1, Mr 110 kDa.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
110 kDa
Histone demethylase that specifically demethylates ′Lys-4′ of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and tri-methylted ′Lys-4′ of histone H3. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 ′Lys-4′ on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. May also demethylate ′Lys-9′ of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.
Immunogen
GST fusion protein corresponding to residues 23-138 of human LSD1.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: CBL770
Physical form
Mouse monoclonal in buffer containing 0.05% sodium azide and 30% glycerol.
Unpurified
Preparation Note
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
10 - Combustible liquids
wgk
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Mohamed-Ali Hakimi et al.
The Journal of biological chemistry, 278(9), 7234-7239 (2002-12-21)
Eukaryotic genes are under the control of regulatory complexes acting through chromatin structure to control gene expression. Here we report the identification of a family of multiprotein corepressor complexes that function through modifying chromatin structure to keep genes silent. The
Dominika Žagar et al.
Journal of applied microbiology, 132(6), 4517-4530 (2022-03-11)
Childcare facilities act as microenvironments that facilitate and promote the selection, spread and transmission of antibiotic-resistant micro-organisms in the community. We focused on the study of antimicrobial resistance and genetic predispositions for β-lactamase production in bacterial isolates from nursery teachers'
Daniella Brasacchio et al.
Diabetes, 58(5), 1229-1236 (2009-02-12)
Results from the Diabetes Control Complications Trial (DCCT) and the subsequent Epidemiology of Diabetes Interventions and Complications (EDIC) Study and more recently from the U.K. Prospective Diabetes Study (UKPDS) have revealed that the deleterious end-organ effects that occurred in both
Lysine-specific histone demethylase 1 inhibitors control breast cancer proliferation in ER?-dependent and -independent manners.
Pollock, JA; Larrea, MD; Jasper, JS; McDonnell, DP; McCafferty, DG
ACS chemical biology null
Daniele Maria Lopes Pinheiro et al.
Free radical biology & medicine, 130, 8-22 (2018-10-27)
Oxidative stress generated during inflammation is associated with a wide range of pathologies. Resveratrol (RESV) displays anti-inflammatory and antioxidant activities, being a candidate for the development of adjuvant therapies for several inflammatory diseases. Despite this potential, the cellular responses induced
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 05-939 | 04053252674914 |
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