Key Documents
05-947
Anti-Phosphotyrosine Antibody, clone PY20
clone PY20, Upstate®, from mouse
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About This Item
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
PY20, monoclonal
species reactivity (predicted by homology)
vertebrates (common species)
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2b
shipped in
wet ice
target post-translational modification
phosphorylation (pTyr)
Gene Information
human ... PID1(55022)
General description
Immunogen
Application
Recommended by an independent laboratory to immunoprecipitate tyrosine phosphorylated proteins.
ELISA:
Recommended by an independent laboratory.
Biochem/physiol Actions
Physical form
Preparation Note
NOTE: DO NOT FREEZE.
For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8°C. Save the supernatant for application.
Analysis Note
Positive Antigen Control: Catalog #12-302, EGF-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected tyrosine-phosphorylated proteins in RIPA lysates EGFtreated human A431 carcinoma cells.
Other Notes
Legal Information
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Global Trade Item Number
SKU | GTIN |
---|---|
05-947 | 04053252673641 |
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