05-988
Anti-Pras40 Antibody, Clone 73P21
clone 73P21, Upstate®, from mouse
Synonym(s):
40 kDa proline-rich AKT substrate, AKT1 substrate 1 (proline-rich), proline-rich Akt substrate, 40 kDa
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Product Name
Anti-Pras40 Antibody, Clone 73P21, clone 73P21, Upstate®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
73P21, monoclonal
monoclonal
species reactivity
human
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... AKT1S1(84335)
Physical form
Purified mouse monoclonal IgG1 in buffer containing PBS with 1% BSA and 0.1% sodium azide.
Affinity purified
Format: Purified
Analysis Note
Control
RIPA lysates of HeLa cells.
RIPA lysates of HeLa cells.
Routinely evaluated by western blot.
Western Blot Analysis:
A 0.1-1.0 µg/mL dilution of this lot detected PRAS40 in RIPA lysates of HeLa cells.
Western Blot Analysis:
A 0.1-1.0 µg/mL dilution of this lot detected PRAS40 in RIPA lysates of HeLa cells.
Application
Anti-Pras40 Antibody, Clone 73P21 is an antibody against Pras40 for use in IP & WB.
Immunoblot Analysis: A 01-1.0 μg/mL dilution of this lot detected PRAS40 in RIPA lysates of HeLa cells.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
PI3K, Akt, & mTOR Signaling
PI3K, Akt, & mTOR Signaling
Biochem/physiol Actions
Recognizes PRAS40.
This antibody does not cross react with mouse.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
40 kDa
PRAS40 (Proline Rich Akt Substrate, 40 kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate as it has the putative Akt phosphorylation motif of RxRxxpS/pT2. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to and regulate mTORC1 (mTOR, Raptor, mLST8) kinases activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it bind to Raptor of mTORC1 in the absence of insulin1. It is thought that through it binding to the Raptor, PRAS40 inhibits the kinase activity of mTORC1 by preventing to bind to its substrates such as p70S6K and 4EBP1.
Immunogen
Recombinant human PRAS40 (full-length) expressed in E. coli.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Preparation Note
Stable for 1 years at -20°C from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Evaluating the therapeutic potential of mTOR inhibitors using mouse genetics.
Huawei Li,Jennifer L Cotton,David A Guertin
Methods in Molecular Biology null
David A Guertin et al.
Cancer cell, 15(2), 148-159 (2009-02-03)
mTOR complex 2 (mTORC2) contains the mammalian target of rapamycin (mTOR) kinase and the Rictor regulatory protein and phosphorylates Akt. Whether this function of mTORC2 is critical for cancer progression is unknown. Here, we show that transformed human prostate epithelial
Daniel W D West et al.
The American journal of clinical nutrition, 94(3), 795-803 (2011-07-29)
Ingestion of whey or casein yields divergent patterns of aminoacidemia that influence whole-body and skeletal muscle myofibrillar protein synthesis (MPS) after exercise. Direct comparisons of the effects of contrasting absorption rates exhibited by these proteins are confounded by their differing
Wenting Mi et al.
Oncotarget, 6(16), 13962-13977 (2015-05-12)
The mTORC1 inhibitors, rapamycin and its analogs, are known to show only modest antitumor activity in clinic, but the underlying mechanisms remain largely elusive. Here, we found that activated AKT signaling is associated with rapamycin resistance in breast and colon
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