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Key Documents

06-1119

Sigma-Aldrich

Anti-TLR2 Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

toll-like receptor 2, Toll/interleukin-1 receptor-like protein 4, toll/interleukin 1 receptor-like 4, CD282 antigen

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse

species reactivity (predicted by homology)

chimpanzee (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology)

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TLR2(7097)
mouse ... Tlr2(24088)

General description

Toll-like receptor 2 (TLR2) is a single-pass type I membrane protein belonging to the Toll-like receptor family and is comprised of 14 LRR repeats and a single TIR domain. TLR2 interacts with LY96 or TLR1 to regulate innate immune response to bacterial lipoproteins and other components of the microbial cell wall. It induces the activation of NFκB, inflammatory response and cytokine secretion through interactions with MYD88 and TRAF6. It may also function as a promoter for apoptosis. High expression of TLR2 has been reported in blood leukocytes, specifically in bone marrow, lymph node, monocytes and spleen. It has also been observed in fetal liver and lung tissues. Modifications in gene expression have been implicated in an increased susceptibility to leprosy.
~90 kDa observed.
Blocking without Tween is recommended to avoid the observance of additional bands.

Specificity

This antibody recognizes the extracellular domain of TLR2.

Immunogen

Epitope: Extracellular Domain
KLH-conjugated linear peptide corresponding to the extracellular domain of human TLR2.

Application

Anti-TLR2 Antibody is an antibody against TLR2 for use in WB, IH(P).
Immunohistochemistry Analysis: A 1:50 dilution from a previous lot detected TLR2 in normal human placenta tissue.
Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
RAW264.7 cell lysate
Evaluated by Western Blot in RAW264.7 cell lysate.

Western Blot Analysis: 2 µg/mL of this antibody detected TLR2 in 10 µg of RAW264.7 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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