06-118
Anti-SHP-2/SHPTP-2 Antibody
Upstate®, from rabbit
Synonym(s):
Tyrosine-protein phosphatase non-receptor type 11, SHP-2/SHPTP-2, Protein-tyrosine phosphatase SYP, SH-PTP2, SHP-2, Shp2
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse
species reactivity (predicted by homology)
rat (based on 100% sequence homology)
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... PTPN11(5781)
General description
64 kDa, apparent size increases to approximately 70 kDa upon tyrosine phosphorylation.
Tyrosine-protein phosphatase non-receptor type 11 (EC 3.1.3.48; UniProt P35235; also known as Protein-tyrosine phosphatase SYP; SH-PTP2, SH2 domain-containing protein tyrosine phosphatase-2, SHP-2, Shp2) is encoded by the Ptpn11 (also known as Syp) gene (Gene ID 19247) in murine species. SHP-2/SHPTP-2 is a non-receptor protein tyrosine phosphatase involved in a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. SHP-2/SHPTP-2 contains two tandem Src homology-2 (SH2) domains that mediate substrates interaction, followed by a protein tyrosine phosphatase PTP domain. Peptide library-based study reveals similar substrate specificities of SHP-2 and the related SHP-1. Both enzymes exhibit preference for acidic and hydrophobic residues flanking the N- and C-terminal sides of target tyrosine residue. Mutations in PTPN11 gene are known to cause Noonan syndrome and acute myeloid leukemia in human.
Immunogen
GST-fusion protein containing residues 2-216 of mouse SHP-2/SHPTP-2
Application
Detect SHP-2/SHPTP-2 using this Anti-SHP-2/SHPTP-2 Antibody validated for use in IC & WB.
Immunocytochemistry Analysis: 4.0 µg/mL from a representative lot detected SHP-2/SHPTP-2 in HeLa, A431, HUVEC, and NIH/3T3 cells.
Research Category
Signaling
Signaling
Research Sub Category
Cytoskeletal Signaling
Cytoskeletal Signaling
Biochem/physiol Actions
Recognizes SHP-2/SHPTP-2.
Physical form
Protein A purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected SHP-2/SHPTP-2 in 10 µg of HeLa cell lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected SHP-2/SHPTP-2 in 10 µg of HeLa cell lysate.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Prolactin enhances insulin-like growth factor I receptor phosphorylation by decreasing its association with the tyrosine phosphatase SHP-2 in MCF-7 breast cancer cells.
Kristopher C Carver,Timothy M Piazza,Linda A Schuler
The Journal of Biological Chemistry null
V C Taylor et al.
The Journal of biological chemistry, 274(17), 11505-11512 (1999-04-17)
The myeloid restricted membrane glycoprotein, CD33, is a member of the recently characterized "sialic acid-binding immunoglobulin-related lectin" family. Although CD33 can mediate sialic acid-dependent cell interactions as a recombinant protein, its function in myeloid cells has yet to be determined.
Specificity of interleukin-2 receptor gamma chain superfamily cytokines is mediated by insulin receptor substrate-dependent pathway.
Xiao, H; Yin, T; Wang, XY; Uchida, T; Chung, J; White, MF; Yang, YC
The Journal of Biological Chemistry null
Thierry Voisin et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 22(6), 1993-2002 (2008-01-17)
Orexins acting at the G protein-coupled receptor (GPCR) OX1R have recently been shown to promote dramatic apoptosis in cancer cells. We report here that orexin-induced apoptosis is driven by an immunoreceptor tyrosine-based inhibitory motif (ITIM) (IIY(358)NFL) present in the OX1R.
M L Vetter et al.
Current biology : CB, 5(2), 168-178 (1995-02-01)
Platelet-derived growth factor (PDGF) promotes mitogenesis in fibroblast cell lines but stimulates neurite outgrowth in PC12 cells that ectopically express the beta PDGF receptor. To determine which substrates must associate with this receptor protein-tyrosine kinase in order to promote neurite
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