Anti-SHP-2/SHPTP-2 Antibody

64 kDa, apparent size increases to approximately 70 kDa upon tyrosine phosphorylation.

Tyrosine-protein phosphatase non-receptor type 11 (EC 3.1.3.48; UniProt P35235; also known as Protein-tyrosine phosphatase SYP; SH-PTP2, SH2 domain-containing protein tyrosine phosphatase-2, SHP-2, Shp2) is encoded by the Ptpn11 (also known as Syp) gene (Gene ID 19247) in murine species. SHP-2/SHPTP-2 is a non-receptor protein tyrosine phosphatase involved in a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. SHP-2/SHPTP-2 contains two tandem Src homology-2 (SH2) domains that mediate substrates interaction, followed by a protein tyrosine phosphatase PTP domain. Peptide library-based study reveals similar substrate specificities of SHP-2 and the related SHP-1. Both enzymes exhibit preference for acidic and hydrophobic residues flanking the N- and C-terminal sides of target tyrosine residue. Mutations in PTPN11 gene are known to cause Noonan syndrome and acute myeloid leukemia in human.

GST-fusion protein containing residues 2-216 of mouse SHP-2/SHPTP-2

Detect SHP-2/SHPTP-2 using this Anti-SHP-2/SHPTP-2 Antibody validated for use in IC & WB.

Immunocytochemistry Analysis: 4.0 µg/mL from a representative lot detected SHP-2/SHPTP-2 in HeLa, A431, HUVEC, and NIH/3T3 cells.

Research Category
Signaling

Research Sub Category
Cytoskeletal Signaling

Recognizes SHP-2/SHPTP-2.

Protein A purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected SHP-2/SHPTP-2 in 10 µg of HeLa cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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