Key Documents
06-1292
Anti-Cep63 Antibody
from rabbit, purified by affinity chromatography
Synonym(s):
Centrosomal protein of 63 kDa
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse
technique(s)
immunocytochemistry: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CEP63(80254)
General description
Centrosomal protein 63 (Cep63) serves an ATM and ATR substrate for spindle assembly activation following chromosomal breakage. It is shown that inactivation of the CEP63 gene in avian DT40 cells impairs spindle assembly and prevents ATM- and ATR-dependent effects on mitosis. Cep63 is also reported to bind to and recruit Cdk1 to centrosomes and is thought to be involved the regulation mitotoic entry, centrosome amplification, and genome maintentance.
~63 kDa observed. Different isoforms or uncharacterized bands may be observed in some endogenous cell lysates. Experiments using transfected cells and siRNA have confirmed detection of Cep63.
Immunogen
Recombinant protein corresponding to human Cep63.
Application
Immunocytochemistry Analysis: A representative lot detected Cep63 in U20S cells. (Nicola Brown, CRUK Clare Hall Laboratories, London, UK)
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Use Anti-Cep63 Antibody (Rabbit Polyclonal Antibody) validated in WB, ICC to detect Cep63 also known as Centrosomal protein of 63 kDa.
Physical form
Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
HEK 293 cell lysate
HEK 293 cell lysate
Evaluated by Western Blot in HEK293 cell lysate.
Western Blot Analysis: A 1: 500 dilution of this antibody detected Cep63 in 10 µg of HEK 293 cell lysate.
Western Blot Analysis: A 1: 500 dilution of this antibody detected Cep63 in 10 µg of HEK 293 cell lysate.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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